小鼠脑皮质神经干细胞体外培养及miRNAs影响其分化能力

目的 系统建立小鼠脑皮质神经干细胞(NSCs)体外培养的技术平台,并探索一组micmRNA对NSCs分化能力的影响.方法 胎鼠(E14)脑皮质分离NSCs进行体外培养;免疫荧光法检测NSCs向神经元和星型胶质细胞的自然分化;Real-time定量PCR筛选一组分化前后表达水平有显著性差异的microRNA;应用新一代脂质体高效转染NSCs,将该组microRNA或其抑制物导人NSCs;Western blot检测导入的RNA序列对NSCs分化能力的影响.结果 基于小鼠NSCs体外培养模型,筛选出一组NSCs分化前后表达水平存在显著性差异的mieroRNA(miR-124,137,128).Western blot结果显示miR-124的反义链能够明显下调β-tubulin Ⅲ的表达(P<0.01),过表达miR-137和miR-128后β-tubulinⅢ的表达量有所升高.结论 脂质体转染技术能够将miRNAs高效导入NSCs中;microRNA-124,137,128能够影响NSCs的分化.     

Choice in Currency in Iceland

An Icelandic post-graduate student at Oxford outlines a method of preventing inflation that could be applied in other countries.     

关于高职院校数学教学中开放题的有关问题初探

本文着重于讲解在高职院校数学教学中常出现的几种开放题,对它们的解法加以研究,归纳其一般思路、解法及其解答技巧,希望能有助于今后高职院校数学教学研究活动,供参考之用。     

Developments in human resource management in manufacturing in modern Britain

In this paper, developments in human resource management in manufacturing in modern Britain based on a national sample are examined. The organization and role of human resource management, employee involvement, training and development, work practices and rewards are all targeted for an assessment of current practices. This is a prelude for a contrast between manufacturing and non-manufacturing. The conclusion is that the similarities between manufacturing and non-manufacturing substantially outweigh the differences. By inference, too, developments in human resource management practices are likely to reflect changes in the broader environment of businesses (and differences between companies) rather than being specific to the main industrial sectors.     

我国电子商务物流模式及选择因素探析

通过分析我国电子商务物流的现状,对当前我国电子商务发展可以选择的物流模式进行分析,最后提出我国电子商务企业进行物流模式选择时所需考虑的因素,以期为我国电子商务企业的发展提供参考.     

我国电子商务物流模式及选择因素探析

通过分析我国电子商务物流的现状,对当前我国电子商务发展可以选择的物流模式进行分析,最后提出我国电子商务企业进行物流模式选择时所需考虑的因素,以期为我国电子商务企业的发展提供参考。     

Trends in multiculturalism in health care

Materiel managers are faced with a shrinking world and changing work environment. The needs and expectations of the customer have shifted because of the increasing number of multicultural groups. These groups will continue to assert their right to culturally sensitive care. Materiel managers also need to learn to support and enhance the skills of a multicultural work force. One important tool for change will be technology. However, technology that brings the world closer together also creates new challenges for managers, who will have to deal with multicultural colleagues, buyers, sellers, and manufacturers of goods and services.     

Explaining Inter-Provincial Inequality in Productivity Growth in Crop Production in Pakistan

Abstract

Data on crop production are assembled for four provinces—Punjab, Sindh, North-West Frontier Province and Balochistan—for the period from 1980/81 to 2005/06 to examine inter-provincial differences in productivity growth rates. Propositions are put forward and examined to explain the superior productivity performance of Punjabi crop producers over those in Sindh and North-West Frontier Province, and the unusual productivity trends in Balochistan. The major factors relate to economic infrastructure and human capital. The impact of agricultural research is difficult to discern, and further work is required to assess the lagged effects of research expenditures on TFP growth.

Explication des déséquilibres interprovinciaux dans l'augmentation de la productivité dans la production de cultures au Pakistan

RÉSUMÉ On a rassemblé des données pour les quatre provinces suivantes: Penjab, Sindh, Khyber-Pakhtunkhwa, et Baloutchistan, pour la période 1980/81 à 2005/06, afin d'examiner les différences interprovinciales dans les taux d'expansion de la productivité. On présente et on examine des hypothèses pour expliquer le rendement supérieur des producteurs du Penjab par rapport à ceux du Sindh et du Khyber-Pakhtunkhwa, ainsi que les tendances de productivité inhabituelles du Baloutchistan. Les principaux facteurs se rapportent à l'infrastructure économique et au capital humain. L'impact de la recherche sur l'agriculture est difficile à établir, et des travaux supplémentaires seront nécessaires pour évaluer les effets décalés des frais de recherche sur l'expansion du TFP.

Explicación de la desigualdad inter-provincial en el crecimiento de la productividad de la producción de cultivos en Pakistán

EXTRACTO Se recopilan datos sobre la producción de cultivos de cuatro provincias: Punjab, Sindh, Provincia de la Frontera Norte-Oeste y Balochistan, durante el período de 1980/81 a 2005/06, para examinar las diferencias inter-provinciales en los índices de crecimiento de la productividad. Se plantean y examinan propuestas para explicar el mayor rendimiento de la productividad de los productores de cultivos Punjabi con respecto a los de Sindh y la Provincia de la Frontera Norte-Oeste, y las inusuales tendencias de la productividad de Balochistan. Los factores principales se relacionan con la infraestructura económica y el capital humano. El impacto de la investigación agrícola es difícil de discernir, y se requiere más trabajo para evaluar los efectos retrasados de los gastos de investigación en el crecimiento TFP.      

坚持以人为本是企业管理之道

随着科学技术的迅速发展,现代企业管理较之过去发生了巨大变化,能不能坚持以人为本的管理,关系到企业能否搞好经营活动,关系到现代企业制度能否顺利建成.     

坚持以人为本是企业管理之道

随着科学技术的迅速发展，现代企业管理较之过去发生了巨大变化，能不能坚持以人为本的管理，关系到企业能否搞好经营活动，关系到现代企业制度能否顺利建成。