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[目的]探讨梅里埃弧菌显色培养基(简称ChromID Vibrio)对霍乱弧菌的检测效果。[方法]通过ChromID Vibrio弧菌显色培养基同硫代硫酸盐-柠檬酸盐-胆盐-蔗糖(简称TCBS)琼脂培养基进行比对,采用阳性菌株直接平板计数、人工污染样品和实际样品检测的方法,对ChromID Vibrio的灵敏性、特异性和检测效果进行了评价。[结果]ChromID Vibrio弧菌显色培养基上霍乱弧菌典型菌落是蓝绿色。阳性菌株直接平板计数及人工污染试验均表明ChromID Vibrio平板的敏感性均比TCBS平板高。对采集的300份实际样品进行检测,都未检出霍乱弧菌。[结论]ChromID Vibrio具有较好的灵敏性、特异性,能提高霍乱弧菌检测效率。  相似文献   
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This study analyses the joint effects of the two transmission routes of cholera on the space‐time diffusion dynamics. Statistical models are developed and presented to investigate the transmission network routes of cholera diffusion. A hierarchical Bayesian modelling approach is employed for a joint analysis of nonlinear effects of continuous covariates, spatially structured variation, and unstructured heterogeneity. Proximity to primary case locations and population density serve as continuous covariates. Reference to communities is modelled as a spatial effect. The study applied to the Kumasi area in Ghana shows that communities proximal to primary case locations are infected relatively early during the epidemics, with more remote communities infected at later dates. Similarly, more populous communities are infected relatively early and less populous communities at later dates. The rate of infection increases almost linearly with population density. A non systematic relation occurs between the rate of infection and proximity to primary case locations. It is discussed how these findings could serve as significant information to help health planners and policy makers in making effective decisions to limit cholera epidemics.  相似文献   
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副溶血性弧菌REP-PCR分型研究   总被引:1,自引:0,他引:1  
[目的]了解食品中分离的副溶血性弧菌REP-PCR分子型别,探讨DiversiLab系统对副溶血性弧菌的分型能力。[方法]采用以REP-PCR为原理的DiversiLab系统对食品中分离的21株副溶血性弧菌进行分子分型,分析菌株之间的相关性,并与PFGE分型结果比较。[结果]DiversiLab将21株副溶血性弧菌分成16个群,菌株之间的相似度64.5%-99.1%,分离自相同食品中的副溶血性弧菌在REP-PCR聚类图中分在同一个群;7株副溶血性弧菌PFGE分型结果产生7种不同的PFGE型别,菌株之间不相关,在REP-PCR聚类图中分在7个不同的群中。[结论]食品中分离的副溶血性弧菌REP-PCR型别分散,未表现出地区特异性;DiversiLab简便、快速、分辨率高,可应用于副溶血性弧菌的快速分型和溯源。  相似文献   
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Vibrio vulnificus has been identified as one of the main causative agents of foodborne disease associated with shellfish consumption. Infections of V. vulnificus increase during the summer months due to higher densities of the bacteria in warmer water and inappropriate handling of shellfish. In Florida, the daily harvest period is regulated to control the length of time between shellfish harvest and processing, and this harvest period has been recently reduced during the summer months to decrease the risk of foodborne disease. Adoption of this public health policy can affect the profitability and economic sustainability of wild oyster harvesters, especially in resource-dependent coastal communities. This study develops a dynamic and stochastic bioeconomic model to assess the impact of this policy on fishers’ harvest and revenues, and weighs that impact against the policy’s potential public health benefits. Our results show that fishers will experience reduced harvests early in the season due to the shorter harvest hours, but this initial loss is partially recouped later in the season as harvests remain high for longer than they would have if the policy were not in place. This study highlights the relationship between food safety interventions and management of fishery resources, and provides a comprehensive framework for evaluating the costs and benefits associated with such interventions.  相似文献   
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目的:弗尼斯弧菌(Vibrio fumissii)是引起世界范围内胃肠功能紊乱的致病菌之一,一般通过摄人生的或者未煮熟的水产品进行传播。本研究旨在建立高效、可靠的PCR检测方法应用于食品中弗尼斯弧菌的快速检测。方法和结果:根据弗尼斯弧菌的toxS基因分别设计普通PCR引物及实时PCR引物、探针,建立普通PCR和实时荧光PCR方法,分别对两种方法的特异性和灵敏度进行比较分析,同时应用于384份食品样品检测。结果表明,建立的普通PCR和实时荧光PCR方法特异性良好,检测灵敏度分别为:2.4×10^3 CFU/ml和24 CFU/ml,并于5 h内完成整个检测过程,同时对384份食品进行检测,共检出6株阳性菌株。结论:本研究建立的PCR检测技术灵敏度高、特异性好,可应用于食品中弗尼斯弧菌的快速检测。  相似文献   
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