| 应用正交设计筛选小叶章诱导培养基的配方 |
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| 引用本文： | 张玉,冷海楠,徐明怡,倪红伟,王丽媛.应用正交设计筛选小叶章诱导培养基的配方[J].国土与自然资源研究,2016(2):87-89. |
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| 作者姓名： | 张玉 冷海楠 徐明怡 倪红伟 王丽媛 |
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| 作者单位： | 黑龙江省科学院自然与生态研究所湿地与生态保育国家地方联合工程实验室,哈尔滨,150040 |
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| 基金项目： | 黑龙江省科学院创新基金 |
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| 摘 要： | 以小叶章的茎段作外植体,采用正交设计法,考察6-BA、TDZ、NAA三种激素对小叶章初代培养产生的影响,确定适宜的激素种类及水平,优化筛选出适宜小叶章的诱导培养基.研究表明,激素6-BA是茎段诱导的关键因素;适宜小叶章初代培养的培养基配方为:1/2MS+6-BA0.5 mg·L~(-1)+TDZ0.1mg·L~(-1)+NAA0.1 mg·L~(-1)。
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| 关 键 词： | 小叶章 正交设计 诱导培养基 |
Selection of Induction Medium for Calamagrostisangustifolia by Orthogonal Design |
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| Abstract: | Young stem from Calamagrostisangustifolia was used as explants. The effects of three kinds of hormones including 6-BA, TDZ, and NAA on Calamagrostisangustifolia were studied. The proper induction medium isselected out by orthogonal design, moreover, the kind and suitable level of hormone were ascertained.The results show that 6-BA is the critical factor for induction, the optimummedium forinitiation is: 1/2MS +6 -BA 0.5 mg·L-1+TDZ0.1mg·L-1+NAA0.1 mg·L-1. |
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| Keywords: | Calamagrostisangustifolia Orthogonal design Induction medium |
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