| 五种血清型柯萨奇病毒的实时荧光RT-PCR检测方法研究 |
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| 引用本文： | 潘良文,黄一,李想,卢钟山,吕蓉,刘月明,高琴,张舒亚.五种血清型柯萨奇病毒的实时荧光RT-PCR检测方法研究[J].检验检疫科学,2010,20(4):1-5,11. |
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| 作者姓名： | 潘良文 黄一 李想 卢钟山 吕蓉 刘月明 高琴 张舒亚 |
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| 作者单位： | 潘良文,李想,卢钟山,吕蓉,刘月明,高琴,张舒亚(上海出入境检验检疫局,上海,200135);黄一(上海出入境检验检疫局,上海,200135;华东理工大学生物工程学院) |
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| 基金项目： | 上海技术标准专项，国家质检总局科研项日，科技部世博科技专项，上海市科委创新平台服务项目项目 |
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| 摘 要： | 在柯萨奇病毒基因组VP1区设计引物和探针,分别建立了特异性检测5种血清型（A9、A16、B2、B3和B5型）柯萨奇病毒（Coxsackievirus）基于MGB探针的实时荧光RT-PCR方法。通过构建的分别适于5种血清型病毒检测的质粒标准分子对建立的检测体系进行了灵敏度分析,确定5种血清型柯萨奇病毒检测体系的检测下限均为2拷贝质粒标准分子DNA。
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| 关 键 词： | 柯萨奇病毒 实时荧光RT-PCR 质粒标准分子 Taqman-MGB探针 |
Research on Detection of Five Serotypes of Coxsackie Viruses with Real - Time RT - PCR |
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| Pan Llangwen,Huang Yi,Li Xiang,Lu Zhongshan,Lv Rong,Liu Yueming,Gao Qin,Zhang Shuya.Research on Detection of Five Serotypes of Coxsackie Viruses with Real - Time RT - PCR[J].Inspection and Quarantine Science,2010,20(4):1-5,11. |
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| Authors: | Pan Llangwen Huang Yi Li Xiang Lu Zhongshan Lv Rong Liu Yueming Gao Qin Zhang Shuya |
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| Institution: | 1. Shanghai Entry - Exit Inspection and Quarantine Bureau, Shanghai, 200135 ;2. School of Bioengineering, Eas China University of Science and Technology) |
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| Abstract: | Based on the VP1 region of Coxsackivirus genome, primer and probe sets have been designed to detect five serotypes of Coxsackieviruses （A9, A16, B2, B3 and B5） separately by real - time RT - PCR. Plasmid standard molecules suitable for coxsaekieviruse A9, A16, B2, B3 and B5 detection were constructed respectively. Limits of detection （LODs） of the developed detection systems for five serotypes of Coxsackieviruse were all 2 copies of plasmid standard molecule DNA. |
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| Keywords: | Coxsackievirus Plasmid Standard Molecule Real - Time RT - PCR Taqman - MGB Probe |
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