| 微生物基因组DNA提取方法的比较与改进 |
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| 引用本文： | 刘晓侠,林建平,岑沛霖.微生物基因组DNA提取方法的比较与改进[J].嘉兴学院学报,2007,19(3):48-50. |
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| 作者姓名： | 刘晓侠 林建平 岑沛霖 |
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| 作者单位： | 1. 嘉兴学院生物与化学工程学院,浙江嘉兴,314001
2. 浙江大学生物工程研究所,浙江杭州,310027 |
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| 摘 要： | 高质量的微生物基因组DNA是基因工程的前提。目前国内外关于微生物基因组DNA提取的方法很多,根据研究对象和目的不同而方法各异。该文就现有方法中应用最为广泛的三种提取微生物基因组DNA的方法进行了比较,并对它们进行一些改进,获得了针对不同细胞壁成分的微生物相应的简便、快速且高质量基因组DNA提取方法,并对提取的DNA进行PCR特异性扩增检测,获得较清晰的谱带1],为基因克隆表达研究奠定了基础。
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| 关 键 词： | 微生物 DNA提取 PCR |
| 文章编号： | 1008-6781（2007）03-0048-03 |
| 收稿时间： | 2006-09-04 |
| 修稿时间： | 2006年9月4日 |
Comparison and Improvement of Extraction Methods for Genomic DNA |
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| LIU Xiao-xia,LIN Jian-ping,CEN Pei-lin.Comparison and Improvement of Extraction Methods for Genomic DNA[J].Journal of Jiaxing College,2007,19(3):48-50. |
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| Authors: | LIU Xiao-xia LIN Jian-ping CEN Pei-lin |
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| Institution: | 1. School of Biology and Chemical Engineering, Jiaxing university, Jiaxing, Zhejiang 3140001 ; 2. Institute of Bioengineering, Zhejiang University, Hangzhou, Zhejiang 310027 |
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| Abstract: | High quality genomic DNA from microorganism is the precondition of genetic manipulation.Now many methods for the extraction of genomic DNA are developed according to different research object and intention.Three kinds of methods widely applied are compared and reformed in isolation of genomic DNA from microorganism.And effective methods for extracting high pure genomic DNA are established considering different cell wall components.Genomic DNA gained by three different methods above is specifically amplified and clear band is observed by agarose gel electrophoresis,which is convenient to genetic manipulation later. |
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| Keywords: | microorganism DNA extraction PCR(Polymerase Chain Reaction) |
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