液相色谱法同时测定防晒霜中16种紫外线吸收剂

建立了同时检测防晒霜中16种紫外线吸收剂含量的高效液相色谱分析方法。样品用甲醇、四氢呋喃、高纯水、高氯酸的混合溶液溶解后超声提取。采用新型ZORBAXSBC18（5tam，250μm×4．6mmi．d．）色谱柱，以甲醇、四氢呋喃、高氯酸水溶液（V／V／V）（pH2．6）梯度洗脱，检测波长311nm。结果显示在22min内16种紫外线吸收利完全分离，在10-4000μg／mL范围内呈线性关系，相关系数大于0．9990；方法定量限为0．02％-0．4％；在市售防晒霜中加标回收率为84．0％．113．9％，相对标准偏差为2．49％．5．75％。本方法简便准确，能够满足进出口检验的需要。

Determination of 16 UV-Filters in Sunscreens by High Performance Liquid Chromatography

A high performance liquid chromatography （HPLC） method was established simultaneous to detect the content of sixteen UV-filters in sunscreens. The samples were dissolved by methanol ＋ THF ＋Ultra-pure water ＋ HCIO4 and extracted by ultrasonic method. The target compounds were separated by a new ZORBAX Eclipse Plus C18column （5pro, 250 mmx4.6 mm i.d.） in gradient elution mode using methanol, THF and aqueous perchloric acid （pH=2.6） as mobile phase, and detected by a photo-diode array detector at 311 nm. The results indicated that the sixteen UV-filters were completely separated in 22 min. The calibration curves was showed good linearity in the range of 10-4000μg/mL, with correlation coefficients larger than 0.9990. The limits of quantitation were in the range of 0.02 to 0.4%. The spiked recoveries of commercial sunscreens were in the range of 84.0% to 113.9% with the relative standard deviation of 2.49% to 5.75%. The results indicated that the method was simple, rapid, accurate and suitable for the requirements in import and export inspection.