LAL／GNB法筛选辐照鸡肉的应用研究

【目的】探讨内毒素检测（LAL）与革兰氏阴性菌计数（GNB）相结合的方法应用于辐照鸡肉的筛选的可能性。【方法】市售鸡肉丁以50g每份分装后，以γ射线进行辐照处理，剂量分别为0kGy、2kGy、6kGy，每个剂量各12份，通过革兰氏阴性菌的培养计数估计样品中存在的活的革兰氏阴性菌的量，通过内毒素浓度定量估计活的和死亡的革兰氏阴性菌总量，比较两者差异来判定是否经过辐照处理。结果】辐照剂量为6kGy时，12个样品被检测出经过辐照处理。辐照剂量为2kGy时，8个样品被检测出经过辐照处理，4个样品检测出未经过辐照处理。辐照剂量为0kGy时，12个样品检测结果均为未经过辐照处理。【结论】LAL／GNB法可以应用干辐照鸡肉的筛选。

A STUDY ON SCREENING OF IRRADIATED CHICKEN BY LAL/GNB

To explore if the method comprising Limulus amoebocyte lysate （LAL） test and enumeration of total Gram-negative bacteria （GNB） can be used for screening of irradiated chicken. Divide diced chicken bought from market into equal parts in 50 gram per package, then irradiate them by different doses of γ -my, each dose including of 12 packages. The applied radiation doses were 0, 2 and 6 kGy respectively. Then determine the number of viable Gram-negative bacteria and the concentration of bacterial endotoxin as a measure for the estimation of the amount of total Gram-negative bacteria, both viable and dead. Compare the differences to decide if the sample had been irradiated. In the group of 6kGy doses, all 12 samples were detected being irradiated; in the group of 4kGy doses, 8 samples were detected being irradiated, while 4 samples were detected not being irradiated. In the group of 0kGy doses, all 12 samples were detected not being irradiated. The method comprising LAL/GNB procedures can be used for screening of irradiated chicken meat