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| 透明颤菌血红蛋白基因克隆及在紫色非硫细菌中的表达 | |
| 引用本文： | 张立国,罗人明,周集体,吕 红,杜进民,郭建博,罗 晓,王晓磊.透明颤菌血红蛋白基因克隆及在紫色非硫细菌中的表达[J].河北工业科技,2008,25(1):1-4. |
| 作者姓名： | 张立国 罗人明 周集体 吕 红 杜进民 郭建博 罗 晓 王晓磊 |
| 作者单位： | 1. 河北科技大学环境科学与工程学院,河北石家庄,050018 2. 大连理工大学环境与生命学院,辽宁大连,116012 3. 河北科技大学生物科学与工程学院,河北石家庄,050018 |
| 摘 要： | 构建了含有透明颤菌血红蛋白基因的可转化大肠杆菌-紫色非硫细菌穿梭质粒(pHSG-MG-vgb),将其转化到紫色非硫细菌(Rhodopseudo palustns),获得了基因工程菌EPB01。经SDS-PAGE和CO示差分光光谱分析证明,重组菌株表达了有活性的透明颤菌血红蛋白,通过低溶解氧诱导后,基因工程菌EPB01的菌体密度较对照菌增加了24%~32%。 |
| 关 键 词： | 透明颤菌血红蛋白基因 天然启动子 光合细菌 基因工程菌 低氧诱导 |
| 文章编号： | 1008-1534（2008）01-0001-04 |
| 收稿时间： | 2007-09-06 |
| 修稿时间： | 2007-11-15 |
Cloning of vitreoscilla hemoglobin gene and expression in Rhodopseudo palustns |
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| ZHANG Li-guo,LUO Ren-ming,ZHOU Ji-ti,L Hong,DU Jin-min,GUO Jian-bo,LUO Xiao,WANG Xiao-lei.Cloning of vitreoscilla hemoglobin gene and expression in Rhodopseudo palustns[J].Hebei Journal of Industrial Science & Technology,2008,25(1):1-4. | |
| Authors: | ZHANG Li-guo LUO Ren-ming ZHOU Ji-ti L Hong DU Jin-min GUO Jian-bo LUO Xiao WANG Xiao-lei |
| Institution: | 1.College of Environmental Science and Engineering;Hebei University of Science and Technology;Shijiazhuang Hebei 050018;China;2.School of Environmental and Biological Science and Technology;Dalian University of Technology;Dalian Liaoning 116012;3.College of Biological Science and Engineering;China |
| Abstract: | An E.coli-Rhodopseudo palustns shuttle plasmid,pHSG-MG-vgb containing vitreoscilla hemoglobin gene was constructed and transformed to Rhodopseudo palustns.As a result genetic Engineering strains EPB01 was obtained.After inducing under low dissolved oxygen conditions,the density of engineering strain increased by 24%～32% compared with that of the control group.The results of SDS-PAGE and carbon monoxide(CO) difference spectrum analysis indicated that the recombinant strain expressed vitreoscilla hemoglobin(VHb) having biological activity. |
| Keywords: | vitreoscilla hemoglobin gene native promoter photosynthetic bacteria engineering strain hypoxia induction |
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