| 克拉维酸高产菌的选育 |
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| 引用本文： | 张汝玲.克拉维酸高产菌的选育[J].河北工业科技,2011,28(3):149-152. |
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| 作者姓名： | 张汝玲 |
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| 作者单位： | 石药集团河北中润制药有限公司,河北石家庄,050041 |
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| 摘 要： | 以带小棒链霉菌bs1574为出发菌株,经γ射线照射,并结合甘油耐受性菌株的理性筛选,选育得到较佳诱变菌株bs2325,效价达2 569μg/mL.采用摇瓶发酵初筛和复筛,选育出甘油耐受性正向突变菌株,是出发菌株(效价为968 μg/mL)的2.65倍,该突变菌株在琼脂斜面培养基上连续转接传代4代,克拉维酸的产量保持稳定...
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| 关 键 词： | 克拉维酸 γ射线诱变 耐甘油 选育 |
| 收稿时间： | 2011/1/10 0:00:00 |
| 修稿时间： | 2011/3/11 0:00:00 |
Breeding of clavulanic acid mutant |
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| ZHANG Ru-ling.Breeding of clavulanic acid mutant[J].Hebei Journal of Industrial Science & Technology,2011,28(3):149-152. |
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| Authors: | ZHANG Ru-ling |
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| Institution: | ZHANG Ru-ling(Hebei Zhongrun Pharmaceutical Company Limited,CSPC,Shijiazhuang Hebei 050041,China) |
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| Abstract: | The method of screening and selecting better mutants of bs2325 by γ-ray irradiation onto streptomyces bs1574 is put forward,and the titer is 2 569 μg/mL.By fermentation screening and secondary screening,strains of mutant glycerol positive tolerance is selected,whose titer is 2.65 times that of the original strain(968 μg/mL).The mutant strain grows well during four generations on the agar slant medium,and the yield of clavulanic acid remains stable. |
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| Keywords: | clavulanic acid γ-radial mutagenesis glycerol-resistant breeding |
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