气相色谱-三重四极杆质谱法测定水产暂养水中丁香酚和异丁香酚

本研究通过使用正己烷饱和的乙腈提取、乙腈饱和的正己烷净化,建立了测定水产暂养水中丁香酚和异丁香酚的气相色谱-三重四极杆质谱法.方法学验证表明,两种麻醉剂在20～500μg·L-1浓度范围内线性良好,检出限为2μg·kg-1,定量限为5μg·kg-1.通过进行3个浓度水平加标,每个浓度平行实验6次.结果表明,两种丁香酚类...

Determination of Eugenol and Isoeugenol in Temporary Aquaculture Water by Triple Quadrupole GC/MS

In this study,a triple quadrupole GC/MS method for the determination of eugenol and isoeugenol in temporary aquaculture water was established byextraction with n-hexane saturated acetonitrile and purification with n-hexane saturated acetonitrile.Methodological verification showed that the two anesthetics had good linearity in the concentration range of 20~500μg·L^-1,with the limit of detection of 2μg·kg^-1 and the limit of quantification of 5μg·kg^-1.Three concentration levels were labeled,and each concentration was tested for 6 times in parallel.The results showed that the recoveries of the two eugenol anesthetics ranged from 85.6%~105.7%,and the relative standard deviations ranged from 1.2%~5.3%.The method is simple,sensitive,accurate and reliable.It is suitable for the determination of eugenol and isoeugenol in temporary aquaculture water.