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| 香石竹斑驳病毒属和潜隐病毒属的PCR检测方法研究 | |
| 引用本文： | 杨雷亮,陈定虎,李明福,陈洪俊. 香石竹斑驳病毒属和潜隐病毒属的PCR检测方法研究[J]. 检验检疫科学, 2009, 19(2): 1-3 |
| 作者姓名： | 杨雷亮 陈定虎 李明福 陈洪俊 |
| 作者单位： | 1. 中山出入境检验检疫局技术中心,广东中山,528400 2. 中国检验检疫科学院,北京 |
| 摘 要： | 根据香石竹斑驳病毒属和香石竹潜隐病毒属主要确定种的外壳蛋白（Coat protein,CP）氨基酸的保守序列分别设计出它们的简并引物,然后对8个香石竹斑驳病毒属的病毒和9个香石竹潜隐病毒属的病毒进行PCR检测验证,结果与预期值相符。 |
| 关 键 词： | 香石竹斑驳病毒属 香石竹潜隐病毒属 PCR |
Detection of Carmavirus and Carlavirus with PCR Method |
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| Yang Leiliang,Chen Dinghu,Li Mingfu,Chen Hongjun. Detection of Carmavirus and Carlavirus with PCR Method[J]. Inspection and Quarantine Science, 2009, 19(2): 1-3 | |
| Authors: | Yang Leiliang Chen Dinghu Li Mingfu Chen Hongjun |
| Affiliation: | Yang Leiliang,Chen Dinghu, Li Mingfu ,Chen Hongjun(1 Inspection Technical Center,Zhongshan Entry - Exit Inspection and Quarantine Bureau, Zhongshan, Guangdong, 528400, China ;2 Chinese Academy of Inspection and Quarantine, Beijing, 100123, China ) |
| Abstract: | The degenerate primers were designed respectively according to the amino acid conserved sequences of coat proteins for Carmovirus and Carlavirus.The validated results showed that the primers were specific for 8 viruses of the Carmovirus and 9 viruses of the Carlavirus in PCR detection,and the PCR products were in accordance with the predicted results. |
| Keywords: | PCR |
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