| 脂肪酶催化的研究进展 |
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| 引用本文： | 刘书来.脂肪酶催化的研究进展[J].化工科技市场,2003,26(4):16-20. |
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| 作者姓名： | 刘书来 |
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| 作者单位： | 郑州工程学院,河南郑州,450052 |
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| 摘 要： | 本文简单介绍了脂肪酶的立体结构和催化机理,综述了非水相的有机溶剂体系中脂肪酶催化的特点与性质,并对微水的无溶剂体系、微乳液体系和超临界体系进行了简单的论述。定向进化技术有助于改善酶的催化特点和应用范围,酶交联晶体的制备和生物印迹方法的采用对提高脂肪酶的活性、热稳定性和选择性也有较大的促进作用。
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| 关 键 词： | 脂肪酶 非水相 定向进化 交联酶晶体 生物印迹 |
| 文章编号： | 1009-4725(2003)04-0016-05 |
| 修稿时间： | 2003年2月8日 |
Advance in Lipase Catalysis |
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| Liu Shulai.Advance in Lipase Catalysis[J].Chemical Technology Market,2003,26(4):16-20. |
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| Authors: | Liu Shulai |
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| Abstract: | The structure and catalytic mechanism of lipase was presented briefly. The characteristics of lipase in the non - aqueous sys-tem( including solvent - free system, reverse - phase micelle and supercritical fluid) were discussed. Directed evolution will improve the catalytic properties and broaden the range of its application. Cross - linked enzyme crystal and imprinting of lipase will be helpful for its catalytic activity, thermal stability and stereoselectivity. |
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| Keywords: | lipase nonaqueous directed evolution Cross - linked enzyme crystal biology imprinting |
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