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| 牛胎盘中免疫调节因子的提取过程研究 | |
| 引用本文： | 牟德华,商宪库,庞书亮,任红,侯占群,郝彦伟,芳闪闪.牛胎盘中免疫调节因子的提取过程研究[J].河北工业科技,2006,23(5):269-270,316. |
| 作者姓名： | 牟德华 商宪库 庞书亮 任红 侯占群 郝彦伟 芳闪闪 |
| 作者单位： | 1. 河北科技大学生物工程与科学学院,河北石家庄,050018 2. 河北沧县防疫站,河北沧县,061000 3. 广西大学轻工与食品工程学院,广西南宁,530004 |
| 基金项目： | 河北省石家庄市科技重大专项项目 |
| 摘 要： | 采用盐析、膜技术及冷冻干燥等手段从牛胎盘中分离提纯了免疫调节因子,并通过大鼠实验验证了提取物的生物活性,效果显著。最佳工艺条件:盐析温度为0℃,pH值为5,转速为6 000r/min,冷冻干燥时间为24 h。在此条件下免疫调节因子提取率为0.673%。 |
| 关 键 词： | 牛胎盘 免疫调节因子 冷冻干燥 纯化 生物活性 |
| 文章编号： | 1008-1534(2006)05-0269-02 |
| 修稿时间： | 2006年3月7日 |
Purification of immunoregulatory factor from cow placenta |
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| MOU De-hu,SHANG Xian-ku,PANG Shu-liang,REN Hong,HOU Zhan-qun,HAO Yan-wei and FANG Shan-shan.Purification of immunoregulatory factor from cow placenta[J].Hebei Journal of Industrial Science & Technology,2006,23(5):269-270,316. | |
| Authors: | MOU De-hu SHANG Xian-ku PANG Shu-liang REN Hong HOU Zhan-qun HAO Yan-wei and FANG Shan-shan |
| Institution: | College of Biology Science and Engineering,Hebei University of Science and Technology,Shijiazhuang Hebei 050018,China;College of Biology Science and Engineering,Hebei University of Science and Technology,Shijiazhuang Hebei 050018,China;Hebei Cangxian Epidemic Prevention Station,Cangxian Hebei 061000,China;College of Light Industry and Food Engineering,Guangxi University,Nanning Guangxi 530004,China;College of Biology Science and Engineering,Hebei University of Science and Technology,Shijiazhuang Hebei 050018,China;College of Biology Science and Engineering,Hebei University of Science and Technology,Shijiazhuang Hebei 050018,China;College of Biology Science and Engineering,Hebei University of Science and Technology,Shijiazhuang Hebei 050018,China |
| Abstract: | Immunoregulatory factor has been purified from cow placenta.The purification steps include salt deposition,film separation,freeze-drying and so on.The bioactivity of extractive has been testified on rats and the result is very good.The optimal extraction condition was that the salt deposition temperature was 0 ℃,the pH value 5 and 6 000 r/min and the freeze-drying time 24 h.In this condition the extraction percent of immunoregulatory factor was 0.673%. |
| Keywords: | cow placenta immunoregulatory factor freeze-drying purification bioactivity |
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