| 毛竹水溶性多糖提取工艺的优化及结构表征 |
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| 引用本文： | 王瓅,王素雅,周芹芹,张万佳. 毛竹水溶性多糖提取工艺的优化及结构表征[J]. 粮食科技与经济, 2021, 46(1): 121-126 |
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| 作者姓名： | 王瓅 王素雅 周芹芹 张万佳 |
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| 作者单位： | 南京财经大学 食品科学与工程学院,江苏 南京 210023;江苏省现代粮食流通与安全协同创新中心,江苏 南京 210023;江苏高校粮油质量安全控制及深加工重点实验室,江苏 南京 210023 |
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| 基金项目： | “十三五”国家重大研发计划(2016YFD060090304);江苏高校优势学科建设工程资助项目(PAPD)。 |
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| 摘 要： | 采用响应面法优化毛竹水溶性多糖的提取工艺,分析其成分,并进行结构表征.研究结果表明,优化的毛竹多糖(BSP)提取条件为:料液比1:27(g/mL)、提取温度78℃、提取时间4 h,该条件下BSP提取率为(5.18±0.07)％,提取物中多糖含量为(72.14±0.02)％,糖醛酸含量为(9.76±0.03)％;BSP具...
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| 关 键 词： | 竹笋 多糖 水提醇沉法 红外光谱 单糖组成 相对分子量 微观结构 |
Optimization of Polysaccharide Extraction from Bamboo Shoot and Its Characterization |
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| Wang Li,Wang Suya,Zhou Qinqin,Zhang Wanjia. Optimization of Polysaccharide Extraction from Bamboo Shoot and Its Characterization[J]. Grain Technology and Economy, 2021, 46(1): 121-126 |
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| Authors: | Wang Li Wang Suya Zhou Qinqin Zhang Wanjia |
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| Affiliation: | (College of Food Science and Engineering,Nanjing University of Finance and Economics,Nanjing,Jiangsu 210023;Collaborative Innovation Center for Modern Grain Circulation and Safety,Nanjing,Jiangsu 210023;Key Laboratory of Grains and Oils Quality Control and Processing,Nanjing,Jiangsu 210023) |
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| Abstract: | Response surface methodology was used to optimize the extraction process of water-soluble polysaccharides from bamboo shoots(Phyllostachys pubescensy),and the components and structure of the extracted polysaccharides were analyzed.The the optimum extraction conditions of bamboo polysaccharide(BSP)were as follows:solid-liquid ratio 27 g/mL,extraction temperature 78℃,extraction time 4 h,the yield of BSP was(5.18±0.07)%.The BSP contained(72.14±0.02)%polysaccharide and(9.76±0.03)%uronic acid.Infrared spectroscopy analysis showed that BSP had the characteristic absorption peaks of acidic polysaccharide.BSP was composed of mannose,glucose,arabinose,xylose,rhamnose,galactose in molar ratios of 62.52∶23.05∶10.35∶2.78∶0.79∶0.51.Gel chromatography showed that BSP was composed of two main peaks with a relative molecular mass of 91.11 and 4.55 kDa respectively,indicating that BSP is a mixed acidic polysaccharide. |
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| Keywords: | bamboo shoot polysaccharide,water extraction and alcohol precipitation method infrared spectrum monosaccharide composition relative molecular weight microstructure |
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