高效液相色谱法同时测定食用油中7种抗氧化剂的方法研究

本研究利用高效液相色谱同时测定食用油中没食子酸丙酯(PG)、没食子酸月桂酯(DG)、没食子酸辛酯(OG)、叔丁基对苯二酚(TBHQ)、叔丁基对羟基茴香醚(BHA)、2,6-二叔丁基对甲基苯酚(BHT)、2,6-二叔丁基-4-羟甲基苯酚(Ionox-100)7种抗氧化剂的测定方法,样品中的抗氧化剂经正己烷溶解、乙腈萃取后,经C18柱分离,乙腈-1.5%乙酸溶液体系为流动相进行梯度洗脱,紫外检测器检测,外标法定量。选择线性范围在1~100mg/L,结果表明7种抗氧化剂呈良好的线性关系,相关系数r大于0.999,方法的测定低限为0.8~2.0mg/kg,回收率在89.3%~110.1%,变异系数在1.5%~4.9%。该方法准确、快速、重现性好,可用于大批量食用油检测中7种抗氧化剂的定量分析。

Determination of 7 Kinds of Antioxidants in Edible Oil by HPLC

The method for simultaneous determination of 7 kinds of antioxidants including propyl gallate(PG),dodecyl gallate(DG),octyl gallate(OG),tert-butylhydroquinone(TBHQ),butylhydroxyanisole(BHA),butylated hydroxytoluene(BHT),2,6-di-tert-butyl-4-hydroxymethylphenol(Ionox-100)by high performance liquid chromatography(HPLC).The antioxidants were extracted by acetonitrile from the samples dissolved by hexane,and they were separated by C18 column with 1.5%acetic acid solution and acetonitrileas mobile phase for gradient elution.The ultraviolet detection and quantitative analysis was achieved by the external standard method.7 kinds of antioxidants had good linear relationship in 1~100mg/L,and the correlation coefficients were above 0.999.The recoveries were from 89.3%~110.1%,and RSD were from 1.5%~4.9%.This method was an accurate,fast,reproducible,which is suitable for simultaneous determination of 7 kinds of antioxidants in edible oils.