高效液相色谱法测定婴幼儿配方乳粉中维生素C含量的FAPAS能力验证结果与分析

建立了一种高效液相色谱法测定婴幼儿配方乳粉中维生素C含量的方法,并分析采用该方法参加FAPAS婴幼儿配方乳粉中维生素C含量测定的能力验证的实验结果.样品经偏磷酸溶液提取后,采用TechMate C18-ST(250 mm×4.6 mm,5μm)色谱柱分离,以甲醇-0.02 mol·L-1磷酸二氢铵水溶液(pH=2.0)...

FAPAS Proficiency Testing Result and Analysis of Vitamin C Determination in Infant Formula by HPLC

To establish a high performance liquid chromatography method for the determination of vitamin C in infant formula and analyze the FAPAS proficiency testing result for vitamin C by the developed method.The samples were ultrasonically extracted with metaphosphate solution and separated on a TechMate C₁₈-ST column(250 mm×4.6 mm,5μm)with the mobile phase consisting of methanol-0.02 mol·L^-1 ammonium dihydrogen phosphate(pH=2.0)(2∶98,V∶V).The flow rate was 0.7 mL·min^-1.The column temperature was set at 25℃and the detection wavelength was 245 nm.The standard curve method was used for the quantitative analysis of vitamin C.According to the proposal method and the ability instructions of FAPAS organization,the sample and the quality control sample were determinated for vitamin C,and“Z”scoring method was used to judge the result.Excellent linearity was obtained in the concentration range of 5.52~88.24μg·mL^-1 for vitamin C with the correlation coefficient(r)of 0.9997.The average recovery was 99.27%with the relative standard derivation(RSD)of 1.17%for vitamin C.Standard addition recovery and researchers comparison were used to control the accuracy of vitamin C content determination. The Z of the capability verification result was 0.1. The method is simple, accurate and reproducible in the detection of vitamin C in infant formula. The result of this verification is satisfactory.