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利用壳聚糖亲和层析提取抑酶肽的研究
引用本文:范继业,张静.利用壳聚糖亲和层析提取抑酶肽的研究[J].河北工业科技,2006,23(3):149-151.
作者姓名:范继业  张静
作者单位:河北化工医药职业技术学院制药工程系,河北,石家庄,050031
摘    要:采用化学改性的壳聚糖为载体,共价法偶联胰蛋白酶,制成抑酶肽亲和吸附剂,将其直接亲和层析牛肺提取液,分离、纯化高比活力的抑酶肽。抑酶肽抑制比活力为71 428 BAEE.mg-1,酶活性回收率为62.5%。该方法质量稳定,成本较低,吸附剂机械强度高,抗污染能力较强,非特异性吸附较小,可反复使用,价格低廉,适于应用。

关 键 词:固定化酶  亲和层析  抑酶肽  壳聚糖
文章编号:1008-1534(2006)03-0149-03
修稿时间:2005年4月15日

New method of preparation of high-purity aprotinin by chemical modified trypsin of chitosan
FAN Ji-ye,ZHANG Jing.New method of preparation of high-purity aprotinin by chemical modified trypsin of chitosan[J].Hebei Journal of Industrial Science & Technology,2006,23(3):149-151.
Authors:FAN Ji-ye  ZHANG Jing
Abstract:Trypsin was covalently linked with chemical modified granule chitosan and was used to isolate and purify aprotinin from the extract of cattle lungs by affinity chromatography.Then high-purity aprotinin was prepared after ultrafiltrating and freeze drying.The result shows: the specific activity of immobilized trypsin on chitosan is 71 428 BAEE/mg,and the activity recovery of trypsin is 62.5%.The purity of aprotinin is high,and it could be used for several times.It is accepted as a simple and stable method suitable for purifying aprotinin with high activity in industrial manufacturing.
Keywords:immobilized trypsin on chitosan  affinity chromatography  aprotinin  chitosan
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