HPLC法测定6-甲基尿嘧啶的含量及有关物质

建立了6-甲基尿嘧啶含量和有关物质的高效液相色谱测定方法。采用C18柱,检测波长为263 nm,流动相为甲醇-0.1 mol/L的醋酸铵(二者体积比为40∶60),流速为1.0 mL/min,进样量为20μL。样品检测质量浓度的线性范围为5～200μg/mL(r=0.999 9),平均回收率为99.42%(RSD值为0.51%)。该方法操作简便、快捷,结果准确、可靠,能有效用于产品的质量控制。

Determination of 6-methyluracil content and related substange in HPLC

High performance liquid chromatography method for analyzing 6-methyluracil and related substance were established.C18 column was adopted at the detection and the detection wavelength was 263 nm.The mobile phase was methanol-0.1mol·L-1 acetate buffer(40∶60,V/V) with flow rate at 1.0 mL·min-1 and the inject volume of sample was 20 μL.The calibration curve of 6-methyluracil is linear in the range of 5～200 μg·mL-1(r=0.999 9),and the average recovery of 6-methyluracil is 99.42%(RSD=0.51%).The method is rapid,accurate and reliable,and it is effective for quality control.