乙型肝炎病毒表位的意义和最新的定量监测方法

PreS1抗原位于乙型肝炎病毒颗粒的外表面,介导病毒-宿主之间的相互作用.是病毒组装和感染侵袭的重要参与因素.对于临床诊断方面的意义在于,它直接反映了血清中HBeAg和HBV DNA的浓度.尤其对HBeAg阴性的病例,有重要的诊断意义.新的荧光定量PCR(FQ-PCR)技术,能够比ELISA技术更快速.灵敏并且能够定量地检测乙型肝炎病毒.将这一技术应用到PreS1基因的检测,将能够一次性检测乙肝病毒的感染数量和复制情况.

Significance and Detection of PreS1 Envelope Antigen of Hepatitis B Virus (HBV)

The PreS1 antigen is located on the outer surface of the HBV virion and judged to be involved in virus-host cell interaction. It is most likely to be an important participant in virus assembly or infectivity. At clinical diagnosis, PreS1 antigen directly reflects the serum concentration of HBeAg and HBV DNA, and simultaneously makes up the diagnostic and therapeutic difficulty because of the absence of HBeAg. To current diagnostic methods of HBV, FQ-PCR defecting gene, a new technology, is more rapid and sensitive than conventional ELISA defecting protein. Af one time if can realize accurate quantification and high degree of reproducibility.