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鲤春病毒核酸检测方法研究进展
引用本文:王姝,张利峰,徐立蒲,高隆英,张振国.鲤春病毒核酸检测方法研究进展[J].检验检疫科学,2012,22(3):49-51.
作者姓名:王姝  张利峰  徐立蒲  高隆英  张振国
作者单位:1. 北京市水产技术推广站,北京,100021
2. 北京出入境检验检疫局
3. 深圳出入境检验检疫局
基金项目:国家重大科学研究计划项目
摘    要:对鲤春病毒核酸检测方法的基本原理和应用情况进行了论述,主要包括RT-PCR、荧光定量RT-PCR、环介导等温扩增技术等,并总结了各方法的特点和发展前景。

关 键 词:鲤春病毒血症  RT-PCR  RT-LAMP

Progress in the Nucleic Acid Testing of Carp Virus
Wang Shu,Zhang Lifeng,Xu Lipu,Gao Longying,Zhang Zhenguo.Progress in the Nucleic Acid Testing of Carp Virus[J].Inspection and Quarantine Science,2012,22(3):49-51.
Authors:Wang Shu  Zhang Lifeng  Xu Lipu  Gao Longying  Zhang Zhenguo
Institution:1.Beijing Aquatic Product Technology Promotion Department,Beijing,100021; 2.Beijing Entry-Exit Inspection and Quarantine Bureau; 3.Shenzhen Entry-Exit Inspection and Quarantine Bureau)
Abstract:In this paper,applications and basic principles of the methods for detecting Spring viraemia of carp were reviewed,including RT-PCR,real-time quantitative RT-PCR,loop-mediated isothermal amplification(LAMP) and etc.The characteristics and prospects of each method were summarized.
Keywords:Spring Viraemia of Carp  RT-PCR  RT-LAMP
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