陕北传统米酒曲中优势菌种的分离、纯化及鉴定

为进一步提高陕北传统米酒的质量,保持或改善其风味,本研究对陕北传统米酒酒曲中的微生物进行分离、纯化、鉴定,并分析了微生物的数量分布。根据培养特性、菌体形态及生理生化检测进行菌种鉴定,得到24个不同属、种的微生物菌株;活菌计数结果显示霉菌和酵母菌为酒曲中的优势菌种,分别占总菌数的57%和38%。将分离所得微生物中17个种的优势菌株进行纯种培养,其中霉菌8株,酵母菌9株。并对8株霉菌所产生的α-淀粉酶和糖化酶活力进行比较研究,结果显示米根霉（Rhizopus oryzae）,华根霉（Rhizopus chinensis）,米曲霉（Aspergillus oryzae）相比另外5株霉菌表现出较高的酶活力,其α-淀粉酶活力分别为798U/g,672U/g,701U/g;糖化酶活力分别为2641U/g,2321U/g,2538U/g,可作为生产米酒的理想菌种。

Separation and Identification of Predominant Strains in Traditional Rice Wine Koji from the North Part of Shanxi

In order to improve the quality and the flavor of the rice wine from the north part of Shaanxi,the microorganisms in the traditional rice wine sake koji have been separated and purified.Their cultural characteristics and morphology were determined,and the physiological and biochemical detections about them were made.The results of the live microbes counting showed that the predominant microorganisms of the sake koji are rhizopus and yeasts,about 57% and 38% of the total microorganisms.17 predominant strains...