响应面法优化超声波辅助提取冬虫夏草多糖工艺研究

为采用响应面优化法对超声波辅助提取冬虫夏草多糖的工艺参数进行优化,设计单因素实验,确定影响提取率的几个因素的大概范围,即超声波提取功率、提取温度、提取时间、提取料液比。然后设计响应面实验对工艺参数进行优化,通过查阅文献,选定蒽酮-硫酸法测定冬虫夏草多糖含量,在490 nm的波长下测定吸光值,算出多糖含量,以此作为响应面设计的响应值。结果表明,超声波辅助提取冬虫夏草多糖工艺参数为超声波功率为300 W,提取时间为30 min,提取温度为45℃,料液比为1/50。在此参数下冬虫夏草多糖提取率最大。

Study on Optimization of Ultrasound Assisted Extraction Technology of Polysaccharides in Cordyceps sinensis by Response Surface Method

In order to use the response surface optimization method to optimize the process parameters for ultrasonic assisted extraction of polysaccharide of cordyceps sinensis, single factor experiments were designed to determine the range of factors affecting the extraction rate, including power ultrasonic extraction, extraction temperature, extraction time, extraction and liquid ratio. Then the response surface experiment was designed to optimize the process parameters, through the literature, the anthrone-sulfuric acid method was chose to determined polysaccharide content of Cordyceps sinensis, the absorbance value was measured at the wavelength of 490 nm, and the polysaccharide content was calculated, as response values of the response surface design. The result showed that the ultrasonic assisted extraction of cordyceps polysaccharide processing parameters were as follows: the ultrasonic power was 300 W, the extraction time was 30 min, extraction temperature was 45 ℃ , and solid-liquid ratio was 1/50. The extraction rate of polysaccharide of Cordyceps sinensis is the largest under these conditions.