黑曲霉发酵生产壳聚糖的研究

研究了黑曲霉发酵生产壳聚糖的培养条件。通过单因素试验和正交试验分析，确定了最佳发酵条件为：培养酵温度30℃、pH值7．0、培养时间72h、摇床转速200r／min。最佳发酵条件下菌体干重达21．38mg／mL。通过电解法、酸碱交替处理提取壳聚糖，壳聚糖产率13．7％，脱乙酰度86．1％。

Study on Production of Chitosan by Fermentation of Aspergillus Niger

The production of chitosan by fermentation of Aspergillus niger was studied. Through single-factor experiments and orthogonal experiment, The optimum fermentation conditions were as follows： the fermentation time was 72 h, rotate speed of fermentation reaction table was 200r/min, pH value was 7, the fermentation temperature was 30℃. Under the optimum fermentation process, the dry cell weight could reach 21.38mg/mL. When chitosan was extracted by electrolysis acid-base method, its deaeetylation degree was 86.1%, yield of chitosan was 13.7%.