HPLC 法测定豆腐黄浆水中有机酸质量浓度

建立利用反相高效液相色谱法同时快速的测定豆腐黄浆水中柠檬酸、苹果酸、酒石酸、草酸、富马酸、琥珀酸6种有机酸的方法．采用Agilent Eclipse XDB-C18色谱柱；紫外检测器，检测波长为210 nm；流动相为甲醇∶0．01 mol/L KH2PO4-H3PO4缓冲溶液（3∶97，pH值为2．85）；流速为0．8 mL/min；柱温为25℃；进样量为20μL．在最佳色谱条件下，柠檬酸、苹果酸、草酸等6种有机酸能同时、快速的分离．线性相关系数r＞0．9824，检出限为0．5558～1．2948μg/mL，相对标准偏差0．13％～4．2％，该方法具有分析速度快，重现性好，精密度高，定量准确等特点．适用于豆腐黄浆水中有机酸的定量分析，对黄浆水开发利用有重要意义．

Determination of organic acids in wastewater of tofu by RP-HPLC

This paper established a method for analysis of citric acid , malic acid, tartaric acid, oxalic acid, fumaric acid and amber acid in wastewater of tofu using reversed -phase high performance liquid chromatography .Agilent Eclipse XDB-C18 column was used as the solid phase （4.6 mm ×150 mm, 5 μm）.The chromatographic conditions were as follows：UV detector wavelength was at 210 nm;mobile phase was methanol：0.01 mol/L KH2 PO4-H3 PO4 buffer solutions （3∶97, pH=2.85）;flow rate was 0.8 mL/min;column tempera-ture was 25℃;sampling volume was 20μL.The results showed that linear correlation coef-ficient r＆gt;0.982 4, detection limits was 0.555 8~1.294 8μg/mL and the relative standard deviation was 0 .13%~4 .2%.This method was simple , sensitive , accurate and reproduci-ble.It was suitable for tofu quantitative analysis of organic acids in the wastewater .