锌指核酸酶在基因组编辑中的应用

基因组编辑是建立在基因靶向修饰的基础上,对生物基因组进行改造的一项新技术。锌指核酸酶(zinc finger nucleases,ZFN)是一种特异性强、应用范围广的人工合成酶,可在靶位点制造DNA双链切口(double-strand break,DSB)进而诱导细胞内源性修复机制,通过同源重组修复(homology-directed repair,HDR)或非同源末端连接(non-homologous end joining,NHEJ)途径实现基因替换和纠正,因而成为基因组编辑的良好工具。本文将就最近文献中提到的锌指核酸酶在基因组编辑中的应用做一综述。

Application of Zinc Finger Nuclease in Genome Editing

Genome editing is a new technology for biological genome transformation based on gene targeting modification.Zinc finger nucleases can induce a double-strand break(DSB) in a specific genomic target sequence,followed by the generation of desired modifications during subsequent DNA break repair,such as replacing or correcting a gene through homology-directed repair(HDR) or non-homologous end joining(NHEJ) pathway,which makes it the excellent tool in genome editing.Thus,I will make a review of application of zinc finger nuclease in genome editing in this article.