| 高效液相色谱法快速测定粮食中赭曲霉毒素A |
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| 引用本文： | 王郑平,;郭健,;曹阳,;尚艳娥,;张品,;李月琪.高效液相色谱法快速测定粮食中赭曲霉毒素A[J].粮食科技与经济,2014(4):31-32. |
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| 作者姓名： | 王郑平 ;郭健 ;曹阳 ;尚艳娥 ;张品 ;李月琪 |
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| 作者单位： | [1]国家粮油质量监督检验中心,北京100162; [2]岛津企业管理(中国)有限公司分析仪器事业部分析中心,北京100020 |
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| 基金项目： | 北京市科学技术委员会项目“粮食及其制品中真菌毒素快速检测技术研究与应用”（Z121100008512001） |
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| 摘 要： | 建立了一种使用岛津超高效液相色谱仪LC-30A测定粮食中赭曲霉毒素A的方法.实验结果表明：在浓度范围0.550 μg/L内,校准曲线相关系数大于0.999;进样量2μL,仪器检出限为0.15 μg/L,定量限为0.5 μg/L.3个浓度下标准品的保留时间和峰面积相对标准偏差分别在0.03％～0.31％和0.41％～1.91％;玉米样品平均加标回收率为88.3％～96.7％.
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| 关 键 词： | 粮食 超高效液相色谱 |
Study on Rapid Detemination of Ochratoxin A in Grain by UPLC |
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| Institution: | Wang Zhengping, Guo Jian, Cao Yang, Shang Yan＇e, Zhang Pin, Li Yueqi (1.National Center for Supervision and Inspection of Grain and Oil, Beijing 100162; 2.Shimadzu (China) Limited ,Analytical Applications Center,Beijing 100020) |
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| Abstract: | A method was established for determining ochratoxin A in grain by high performance liquid chromatography.The linear detection ranges of ochratoxin A were 0.5-50μg/L with correlation coefficients of 0.999.The retention volume of ochra- toxin A was 2μL,the detection limit was 0.15μg/L,and the limit of quantification was 0.5μg/L.The relative standard deviations of retention time and peak area under three concentrations were between 0.03% to 0.31% and 0.41% to 1.9 l%.Recovery rates in corn spiked with ochratoxin A were between 88.3% to 96.7%. |
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| Keywords: | Ochratoxin A grain High Performance Liquid Chromatography |
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