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响应面法优化麦胚多糖的醇析工艺
引用本文:桂志远,王学东.响应面法优化麦胚多糖的醇析工艺[J].粮食科技与经济,2011,36(1):54-56.
作者姓名:桂志远  王学东
作者单位:武汉工业学院,食品科学与工程学院,湖北,武汉,430023
摘    要:采用水提醇沉法提取麦胚多糖,将提取液浓缩至合适体积再加入不同浓度的乙醇,利用多糖不溶于乙醇的特性将其沉淀出来,采用苯酚一硫酸法测定醇析物中多糖含量,并用相关性检验分析了醇析物质量和多糖含量的相关性,皮尔逊相关系数r=-0.884〉rmin=0.468,说明两者之间存在显著的相关性。为了提高麦胚多糖醇析工艺中多糖含量,通过Box—Behnken中心组合设计(CCD)及响应面分析(RSM),以醇析物为响应值建立了预测多糖含量的二次多项数学模型,优化了多糖醇析工艺,为现实生产提供可靠的理论依据。

关 键 词:麦胚多糖  醇析工艺  相关性  响应面

Study on Optimal Response Surface Method for Wheat Germ Polysaccharides Alcohol Precipitating Technology
Gui Zhiyuan,Wang Xuedong.Study on Optimal Response Surface Method for Wheat Germ Polysaccharides Alcohol Precipitating Technology[J].Grain Technology and Economy,2011,36(1):54-56.
Authors:Gui Zhiyuan  Wang Xuedong
Institution:Gui Zhiyuan,Wang Xuedong(College of Food,Wuhan Polytechnic University,Wuhan,Hubei 430023)
Abstract:Wheat germ polysaccharide(WGP) was extracted by the method of water extracting-alcohol precipitating.Wheat germ extract will be concentrated to appropriate volume,then different concentrations of ethanol was added to precipitate polysaccharide which was insoluble in it.Phenol-sulfuric acid was used to determinate the content of polysaccharide and according to the correlation test,there was a close correlation between the precipitates and WGP.In order to increase the content of WGP,using precipitate as the r...
Keywords:wheat germ polysaccharide(WGP)  alcohol precipitating technology  correlation test  RSM  
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