响应面法优化超声波辅助提取瓜蒌籽蛋白工艺研究

以瓜蒌籽为原料,利用响应面法优化超声波辅助碱溶酸沉法提取瓜蒌籽蛋白工艺条件.在单因素实验基础上,设定超声工作/间歇时间为2s/1s、pH值为10,选取料液比、超声功率、超声时间、提取温度为影响因子,以瓜蒌籽蛋白提取率为响应值,应用Box-Behnken中心组合试验设计建立数学模型,进行响应面分析.得出提取工艺优化条件为：料液比1：32（g/mL）、超声波功率310W、超声时间39 min、提取温度48℃.在此条件下,瓜蒌籽蛋白提取率可达79.11％.

Optimizing on Ultrasonic-Assisted Extraction of Protein from Trichosanthes Kirilowii Seeds by Response Surface Methodology

Using the Trichos anthes kirilowii seeds as raw material, the ultrasonic-assisted extraction processes of protein were optimized from Trichosanthes kirilowii seeds by response surface methodology.Based on single factor experiment,and setting the ultrasonic running and interim time 2 s/1 s and pH 10,chose the material-liquid ratio, ultrasonic power, ultrasonic time and the extraction temperature as impact factors, and the yield of protein as response value.Mathematical model was set up by Box- Behnken experimental design for response surface analysis.The optimal extracting conditions by the response surface analysis were showed as followed： that the optimum extraction conditions of ultrasonic aided extracting were as follows ：material-liquid ratio 1：32 （g/mL）, ultrasonic power 310 W, ultrasonic time 39 rain and extraction temperature 48 ℃.Under the conditions, the yield of protein from Trichosanthes kirilowii seeds could reach 79.11%.