吐温80在油脂类调味品中微生物检测的研究分析

通过对牛肉酱、红油2种即食油脂类调味品进行微生物检测,将不同比例的吐温80添加到样品前处理的稀释液中,采用浓度为107 CFU·mL-1的标准菌悬液,用常规细菌培养法检测结果。结果表明:选用比例为5%的吐温80对牛肉酱、红油的微生物指标进行检测,检测结果均在107 CFU·mL-1,与空白对照的检测结果在同一数量级上。与常规的稀释液相比,吐温80可使样品均匀分散在稀释液中,且不影响微生物的检测结果,可适用于高油脂类样品的日常微生物检测。

Research and Analysis of Microbial Detection of Tween-80 in Oils and Fats Condiments

Through the microbial detection of two kinds of instant oil condiments,beef sauce and red oil,the different proportion of Tween 80 was added to the diluent of sample pretreatment,the standard bacterial suspension with concentration of 107 CFU·mL-1 was used,and the results were detected by conventional bacterial culture method.The results showed that the ratio of Tween 80 was 5%to detect the microbial index of beef sauce and red oil,and the results were all in the range of 107 CFU·mL-1 which was the same order of magnitude as the blank control.Compared with conventional diluent,Tween-80 can disperse the sample in diluent without affecting the result of microbial detection,and can be used for routine microbial detection of high-fat samples.