QuEChERS结合液相色谱-串联质谱法快速测定鸡肉中磺胺类残留

采用QuEChERS方法提取和净化鸡肉样品,建立测定磺胺类12种兽药残留的液相色谱-串联质谱(LC-MS/MS)检测方法。样品经乙腈和缓冲盐包SBEQ-CA8802-B提取,提取液经净化管净化后取上清液,旋转蒸发仪减压蒸干,溶解过膜,在电喷雾离子源多反应监测(MRM)模式下进行测定,基质外标法定量。结果表明,12种磺胺类药物测定方法的线性范围为0.01~0.20μg·kg^-1,线性相关系数r均大于0.999,定量检出限为0.02~0.12μg·kg^-1,10μg·kg^-1添加范围的平均回收率为87.6%~101.0%,相对标准偏差为3.53%~11.60%。该方法操作简单,净化效果好,灵敏度高,能够快速有效地检测鸡肉中的磺胺类药物残留。

Quick Determination of Sulfonamide Residues in Chicken Meat by QuEChERS Combined with LC-MS/MS

The QuEChERS method was used to extract and purify chicken samples,and an LC-MS/MS detection method was established to determine the residues of 12 sulfa veterinary drugs.The sample is extracted with acetonitrile and buffer salt packet SBEQ-CA8802-B,the extract is purified by the purification tube and the supernatant is taken,and the rotary evaporator is evaporated to dryness under reduced pressure,dissolved through the membrane,and in the electrospray ion source multiple reaction monitoring(MRM)mode Measured under the matrix external standard method for quantification.The results show that the linear range of the 12 sulfonamides determination methods is 0.01~0.20μg·kg^-1,the linear correlation coefficient r is all greater than 0.999,and the quantitative detection limit is 0.02~0.12μg·kg^-1,10μg·kg^-1 the average recovery rate of the addition range is 87.6%~101.0%,and the relative standard deviation is 3.53%~11.60%.The method has simple operation,good purification effect,high sensitivity,and can quickly and effectively detect sulfa drug residues in chicken meat .