曲酸摇床发酵与发酵罐发酵工艺参数的比较

通过单因素及正交实验,比较高产米曲霉在摇床培养与发酵罐培养中生产曲酸的发酵工艺参数。结果表明,在温度为23℃、发酵天数为9 d、接种量为25%、菌龄为96 h时,摇床发酵的曲酸产量达到最高,最高可达98.6 g·L-1;而发酵罐培养的最佳工艺条件是温度23℃、发酵天数4 d,接种量15%,菌龄96 h时,此条件下曲酸产量可达到99.6 g·L-1。本研究为工业化生产曲酸提了供理论依据。

The Comparison of Kojic Acid Fermentation Process Parameters between Shaker Cultivation and Fermentor Cultivation

The fermentation parameters of aspergillus oryzae were compared by single factor and orthogonal experiment.The results showed that when the temperature was 23℃,the fermentation days were 9 d,the inoculum was 25%,and the age of bacteria was 96 h,the yield of kojic acid was the highest,up to 98.6 g·L-1.The optimum technological conditions for fermentation tank culture were 23℃,fermentation days for 4 days,inoculation amount of 15%,and bacterial age of 96 h,under which the yield of kojic acid could reach 99.6g·L-1.This study provides a theoretical basis for industrial production of kojic acid.