DNA条形码在濒危动物鉴定中的应用及进展

DNA条形码技术作为一种有效的分子生物学技术,在物种的精确鉴定中发挥着重要作用.本文概述了近年来DNA条形码技术,特别是DNA宏条形码技术在濒危动物物种鉴定中的研究进展及应用,旨在为物种精确鉴定新技术、新方法的研究提供参考,以及为海关、林业、公安等部门打击野生动物走私、非法贸易取证提供技术支持.     

基于ITS序列的DNA条形码技术鉴定罂粟科种子

采用基于ITS4/ITS5序列的DNA条形码分子生物学技术,结合形态学和地理分布信息,对满洲里口岸进境俄罗斯粮食中检出的2种未知种子进行检疫鉴定.结果表明,2种未知种子分别为红花疆罂粟[Roemeria refracta(Stev.)DC.]和长荚罂粟(Papaver dubium L.).本方法具有快速、简便、准确的...     

应用DNA条形码技术鉴定口岸截获的国内未见分布蚊种

[目的]利用DNA条形码技术对口岸截获的形态特征破坏严重的未知蚊类进行种类鉴定。[方法]提取从荷兰进口的废纸中截获的一头形态严重破坏的蚊类的基因组DNA。采用无脊椎动物线粒体细胞色素氧化酶I（COI）的通用引物LCO1490和HCO2198扩增目的片段。然后纯化、克隆测序、序列分析、与BOLD中的序列进行比对。[结果]该未知蚊类的DNA条形码序列与BOLD中环带林蚊（Sylvicola cinctus）的序列相似性超过99.8%;并与原产地专家交流确认截获的蚊种为环带林蚊。经科技查新,确认为首次截获的国内未见分布蚊种。[结论] DNA条形码能克服传统物种鉴定依靠完整的外部形态的局限,有效提高外来物种的种类鉴别能力,大大缩短检测周期,降低外来物种对我国生态和公共卫生安全的危害。     

燕麦属杂草种子鉴定方法的比较研究

本文通过对燕麦属杂草种子鉴定方法的研究,获得一种快速而有效的燕麦属杂草种子鉴定方法。通过对截获的6种燕麦属杂草种子的形态特征和分子方法鉴定进行研究,形成新的检索和形态特征表,结合分子方法发现psbA-trnH引物对燕麦属杂草种子的鉴定快速而有效。研究表明,海关截获的杂草种子可以通过形态特征和DNA条形码技术进行鉴定。该研究为口岸杂草种子快速检疫鉴定提供了参考依据。     

应用DNA条形码技术快速鉴定入境船舶上截获的昆虫蛹

[目的]利用DNA条形码技术快速准确鉴定从入境船舶上截获的未知双翅目昆虫蛹。[方法]将截获的5只蛹,1只蛹直接冷冻,4只孵化出成蝇;对蛹、蛹壳及孵化出的成蝇进行拍照留档。分别从蛹、蛹壳和孵化成蝇中提取基因组DNA;采用动物DNA条形码通用引物（LCO1490和HCO2198）扩增目的片段,并进行纯化、克隆测序和序列分析,与NCBI及BOLD中的序列进行比对,构建NJ树;对孵化成蝇进行形态鉴定。[结果]所检测的蛹、蛹壳以及孵化成蝇的DNA条形码序列与NCBI和BOLD中大头金蝇（Chrysomyia megacephala）的序列相似度达100%;孵化成蝇与大头金蝇的形态特征相符。[结论]根据测定结果,判定截获的未知双翅目昆虫蛹为大头金蝇的蛹;证明DNA条形码技术是一种鉴定未知昆虫蛹的有效手段。对于口岸截获的外来未知昆虫蛹、成虫残肢甚至蛹壳均可直接采用DNA条形码技术进行种类鉴定,大大缩短鉴定周期。     

条码技术在移动电子商务领域的应用现状及前景

条形码技术是在计算机的应用中产生和发展起来的一种自动识别技术。它是为实现对信息的自动扫描而设计的,是快速、准确、而可靠的采集数据的有效手段。条形码技术的应用解决了数据输入和数据采集的“瓶颈”问题。十多年来,以条形码技术为代表的自动识别技术,很快便渗透到计算机管理的一些领域。条形码技术以从商业零售领域向运输、物流、电子商务和产品追溯等多领域拓展,并带动了条码产业的产生和发展。     

检疫性杂草分子鉴定研究进展

综述了限制性内切酶片段长度多态性、随机扩增多态性、扩增片段长度多态性、简单重复序列、单核苷酸多态性等分子标记技术及DNA条形码技术在检疫性杂草鉴定研究中的应用,分析了各个技术的主要特点,阐述了各个技术的应用情况,并对分子标记及DNA条形码技术在检疫性杂草鉴定中的发展提出展望。     

基于条形码的图书管理系统设计

20世纪80年代条形码技术应用在各种商品中，大大的简化了基本数据的采集工作。同时国家的印刷业快速发展使得图书业技术得以革新，在此是时条形码技术广泛应用在图书上。传统的手工管理图书已经不在适用，但物联网新兴技术发展还不成熟的今天，如果用它来管理图书不但不方便，而且还增加了很多的工作量。本文基于条形码技术设计用于管理高校中图书，其投入低、使用方便、工作量底是最大的特点。     

数据挖掘:零售企业提升竞争力的利器

随着条形码技术、数据库技术的普遍应用,零售企业积累着越来越庞大的交易数据,这些数据背后隐藏着单靠数据库技术无法发现的有关消费者行为模式的重要信息。作为一种从数据中获取知识的工具,数据挖掘能从海量交易数据中抽取分类、聚类、关联、序列模式等关系,组合成顾客的消费行为模式,完成从已知的交易数据中寻找出未知的顾客消费行为模式这一全新的知识的过程,从而提升零售企业的竞争力。     

二维条码技术在外资登记管理中的应用

二维条码技术是在计算机的应用实践中产生和发展起来的一种自动识别技术。超市中常用的带有条形码识读器的收款机,运用的是一维条码技术。二维条码储存的信息量要大大超过一维条码,可以快速、准确地进行数据采集输入,解决了手工数据输入速度慢、错误率高等造成的“瓶颈”难题。     

Brown＇s Blossom in Britain-China

The overall strategic relationship between U.K. and China is ＂better than ever＂, China- Britain relations have maintained good momentum, with increased high-level exchanges and the reinforcement of strategic mutual trust, with more room for growth in the future.     

Challenge and Opportunity Go Hand in Hand——What Global Economic Agitation Brings to the China's Economy?

Domino effects of the global finance agitation As the origin of the subprime crisis,US financial institutions lost USS45 billion last year,accounted for over half of the total US,EU & Japan loss.Since January 15th,Chtigroup released its financial statement of the 4th quarter,showing a net deficit of USS9.83 billion,which marked the hisoric high of its quarterly deficit.     

Sinosteel：Extraordinary Moves in Industry Chess

Among the Fortune 500 firms,more and more images from China emerge out.On the 2008 list,Sinosteel Corporation,a landmark group of China industries quietly squeezes into the candidate line.As the largest steel service ＆ trade enterprise,its sales reached at RMB 111.24 billion,with a year-on-year increase of 83.03 percent,and the profit grew by 180.03 percent.On the Sinosteel 2008 Work Meeting,the president Huang Tianwen uttered,＂In the coming year,we will work for US$20 billion sales,and look forward to getting a position in Fortune 500 firms for ourselves.＂     

Walk Through Unusual Five Years—— An Exhibitor＇s Canton Fair Course

When the 103rd Canton Fair is approaching, an old friend of Canton Fair, Li Ying from Jiangsu prov-ince recollected his past five years＇ experiences to the grand trade meeting. Each year is special, with some unforgettable memories colored by the year.     

Jean-Pierre Duprieu： Willing to Be A Legate of Sino-French Business

Estaing, former French president, Valery Giscard d＇Estaing attended Sino-French Economic Seminar held in Shanghai in early April and said that the world center has shifted from the west to the east with many European and US companies entering into Asia, and China has the ability to play an important role in the world stage. Estaing, naming himself＂French Confucian＂, then said in Chinese that a cook-group includes 10 people in ancient Chinese soldier system and the cook is in charge of cooking for them. And people who belong to the same cook are actually ＂Partners＂.     

China''''s Stock Market Wave

The annual meeting of the World Economy Forum was held in Davos,a little town in Switzerland,on January 23rd to the 27th this year,with the theme"the Power fo Collaborative Innovation",over 2500 celebrates from ture political and cconomic circles of more than 80 countries and challengred logether to talk about the future of the global economy in 2008,A Forum for the problems and challenges that face whole world,some of the keywords which drew attention during the event were:the U.S.subprime mortgage crisis,soaring energy prices,the economic slowdown in the U.S.ermerging economic bodies,authority funds and the general unpredictable nature of the global economy.     

Trade Promotion from Neighborhood

If you are considering to trade with companies in Japan or expanding your .business to Japan, we are ready to help from the very beginning.＂ You will find this greeting at the first sight on the Chinese website of Japan External Trade Organization （JETRO）. JETRO has committed itself in delivering highly efficient and quality services to meet the demand of internal and external customs including Japanese companies, hence winning a good reputation in the international trade arena. In recent years, with its priority in advancing business cooperation in South and East Asia, JETRO has made great achievement in promoting trade between China and Japan. Therefore, China＇s Foreign Trade interviewed Mr. Yoichi Maine, Deputy Director General of JETRO BEIJING.     

Auto Parts Market Potential Coming into Surface

Looking at the increasingly fierce competition in the world auto market. global automobile manufacturing industry, taking Europe, the U.S. and Japan as representatives, is gradually forming a new type of relationship, mutual cooperation between whole vehicle and parts manufacturers. We can clearly see that auto parts manufacturers have become independent from the whole vehicle manufacturing companies, which means that the former structure of auto industry has greatly changed, and a new relationship,     

Public Financial Expenditure——A Reflection of the Livelihood Improvement

In recent years.China's public finance put social construction high on the agenda and attaches more importance on livelihood insurance and improvement.The 2003-2007 public financial expenditure reflects a fact that the government isputting more emphasis on livelihood andissues concerning the people.     

Voices from the MPC and CPPCC

"Crucial for economic growth.energy utilization has its role in all economic sectors and every aspect of people's daily life.Now we are in dire need of a new profession,namely energy manager.We not only need to have passion and responsibility,but also a scientific approach to conserve energy and cut emissions.APpropriate legislation should be in place.and a special profession should be introduced to ensure the implementation of these laws and regulations."