显色培养基分离婴幼儿乳粉中阪崎肠杆菌的效果比对和应用

分析广东环凯公司阪崎肠杆菌显色培养基(CRM006)和科玛嘉阪崎肠杆菌显色培养基(DFI)的实验应用效果,为检测婴幼儿乳粉中的阪崎肠杆菌选用培养基提供依据。按照《食品卫生微生物学检验阪崎肠杆菌检验》(GB/T 4789.40-2010)分离及生化鉴定阪崎肠杆菌,并分析培养基的分离效果。结果表明,36份样品中,检出阪崎肠杆菌2份,检出率为5.56%;CRM平板和DFI平板阳性符合率为100%;疑似菌落检出率分别为16.67%和13.89%(P0.05)。CRM平板和DFI平板用于培养分离阪崎肠杆菌均有良好效果。     

检测单增李斯特氏菌的几种选择性固体培养基的效果比较

[目的]选择更加适合单增李斯特氏菌检测的固体选择性培养基.[方法]参见ISO/TS11133-2中的培养基验证方法并有所改进,对国际上常用的3种选择性固体培养基进行比较选择.[结果]OXA和李斯特氏;显色培养基的增菌效果相近,且单增李斯特氏菌和绵羊李斯特氏菌在李斯特氏菌显色培养基上具有特征性菌落形态,易于与其他李斯特氏菌相区别.[结论]OXA和李斯特氏菌显色培养基效果较好,比较适合单增李斯特氏菌的检测,两种培养基联合使用可以提高单增李斯特氏菌的检出率.     

4种检测技术对冷冻食品沙门氏菌检测效果的比较

[目的]探讨不同检测技术在冷冻加工食品沙门氏茵检测中的实际应用的适用性.[方法]利用Reveal 抗体夹心技术,Vidas自动酶标免疫测试仪、DNA杂交探针技术和SN常规培养生化技术对4008份加工冷冻产品和11株菌种进行检测.[结果]Reveal测试条阳性数151,阳性率3.77%,无假阴性,假阳性数133;Vidas测试条阳性数27,阳性率0.67%,假阴性数3,假阳性数12;DNA杂交探针试剂盒阳性数18,阳性率0.45%,无假阴性,假阳性数1;SN培养法阳性数15,阳性率0.37%,假阴性数3,无假阳性.[结论] Reveal试剂盒敏感性高,但特异性不强,操作简便,所需材料、设备少、适于现场和多样品批量检验;Vidas自动酶标免疫测试仪有假阴性,说明有漏检可能,SN培养法结果可靠,但耗时长、操作繁琐;DNA杂交探针试剂盒法敏感特异,准确性高,适用于对准确性要求较严行业的检验.     

进出口食品中沙门氏菌的高效检测

本文简述了当前沙门氏菌检验方法,总结了广州出入境检验检疫局食品实验室在进出口食品沙门氏菌检测中有针对性地采用多种手段配合的检验方法,以及实现简化检测过程、提高检测阳性率的实践经验,同时提供了近年来在进出口食品中检出的沙门氏菌事例,以期为食品中沙门氏菌的检测提供参考.     

弧菌显色培养基（ChromID Vibrio）对霍乱弧菌检测效果的探讨

[目的]探讨梅里埃弧菌显色培养基（简称ChromID Vibrio）对霍乱弧菌的检测效果。[方法]通过ChromID Vibrio弧菌显色培养基同硫代硫酸盐-柠檬酸盐-胆盐-蔗糖（简称TCBS）琼脂培养基进行比对,采用阳性菌株直接平板计数、人工污染样品和实际样品检测的方法,对ChromID Vibrio的灵敏性、特异性和检测效果进行了评价。[结果]ChromID Vibrio弧菌显色培养基上霍乱弧菌典型菌落是蓝绿色。阳性菌株直接平板计数及人工污染试验均表明ChromID Vibrio平板的敏感性均比TCBS平板高。对采集的300份实际样品进行检测,都未检出霍乱弧菌。[结论]ChromID Vibrio具有较好的灵敏性、特异性,能提高霍乱弧菌检测效率。     

利用PCR方法快速检测食品中的沙门氏菌

本文介绍了一种快速检测食品中沙门氏菌的方法.操作步骤为,在缓冲蛋白胨水(BPW)中增菌18-24h,生理盐水洗菌2次后煮沸裂解细胞,以invA为目的基因进行PCR,琼脂糖凝胶电泳检测,两天时间即能得到明确结果.以全脂奶粉、生牛肉和加工过的鸡肉为实验对象,人为添加沙门氏菌,检测结果与传统培养方法和BAX(r)系统检测结果一致.实验检出限为100cfu/25g,准确性达100%.     

O9群沙门氏菌胶体金检测试剂盒的研制

[目的] 为快速检测O9群沙门氏菌研制检测试剂盒.[方法] 应用胶体金免疫层析法研制O9群沙门氏菌胶体金检测试剂盒,并与国标法进行水平测试.[结果] 该试剂盒测定的结果与国标法基本一致,且该试剂盒具有灵敏度高,特异性强,无交叉反应,精密准确.[结论] 可用于食物中毒流行病调查、食品卫生、动物疫病监测中的快速检测.     

检测致病性李斯特氏菌的培养基

要检出单核增生李斯特氏菌,前增菌和分离是非常重要的步骤。本文概述了李斯特氏菌选择性增菌肉汤和选择性显色培养基的种类和设计原理,对它们的应用效果进行了讨论。     

牛人文化撬动主流媒体镜头

悉数优酷代表的视频行业蓬勃发展的3年历程,从06年“一个馒头引发的血案”的“恶搞”风潮到08年“山寨文化”的流行,我们大众的娱乐方式经历着明星八卦到名人热潮的转变轨迹。     

Notes to self: the visual culture of selfies in the age of social media

This paper explores the cultural fascination with social media forms of self-portraiture, commonly known as “selfies,” with a specific interest in the self-imaging strategies of young women in their teens and early 20s. Ubiquitous on social media sites like Facebook, Tumblr, Flickr, and Instagram, the selfie has become a powerful means for self-expression, encouraging its makers to share the most intimate and private moments of their lives – as well as engage in a form of creative self-fashioning. Popularly regarded as a shallow expression of online narcissism, the selfie is both adored and reviled; yet it flourishes as one of the most effective outlets for self-definition. Through a critical engagement with a history of feminist representational politics, this paper explores the political urgency at the heart of the selfie phenomenon, and contemplates whether the urge to compulsively self-image is mere narcissism, or a politically oppositional and aesthetic form of resistance.     

Expatriate management: Comparison of MNCs across four parent countries

This article reports the results of a study of expatriate management and headquarters‐subsidiary relations in 29 American, British, German, and Japanese multinationals and a sample of 46 of their foreign subsidiaries based on face‐to‐face and telephone interviews with key international HR, subsidiary HR, and subsidiary managing directors. We found that earlier studies, heavily weighted with U.S. multinationals, cannot necessarily be applied to expatriate management experiences of other national industrial countries. Also, expatriate management is more similar for American and British MNCs, while both German and Japanese multinationals in our sample had fairly distinct systems of using expatriates in their foreign subsidiaries. Thus, we can discuss at least three fairly distinct models of expatriate management and corporate‐foreign‐subsidiary control. © 2000 John Wiley & Sons, Inc.     

The effects of benefit-driven commitment on usage of social media for shopping and positive word-of-mouth

Shopping on social media (SM) is more popular now than ever. SM enables interactive communication among users and provides great opportunities for marketers and advertisers to reach out to consumers. While SM has potential as a marketing platform, not much is known whether consumers perceive benefits of SM as a shopping platform and how such perceived benefits can drive their shopping activity on SM. The purpose of this study is to examine the relationship between consumers’ multi-dimensional perceived SM benefits, their commitment to SM, the actual usage of SM for shopping purposes, and their willingness to spread positive comments about their shopping experience on SM. Results of an online survey (N = 325) successfully confirmed the proposed model: consumers perceive functional, experiential, and/or symbolic benefits from using SM, show calculative, affective, and/or normative commitments, use SM for shopping purposes, and engage in generating positive electronic word-of-mouth. This research contributes to the literature by revisiting SM as an emerging shopping platform and empirically validating the conceptual model that lays out the motivational processes underlying shopping via SM.     

The unbearable lightness of advertising: culture,media and the rise of advertising in socialist Bulgaria

This article offers a historical analysis of the conceptualization of advertising both as a propaganda tool and as a means of encouraging consumption in socialist Bulgaria. By exploring the public discourse on the function and importance of advertising in the popular media over the last 50 years, it seeks to explore the approach used to define the need for advertising, offering an insight into the complex ways in which socialist ideologies recognized the “inevitable” logic of the market – on one hand, acknowledging the need for consumption and on the other, recognizing the utility of advertising, which promotes and supports this very consumption as it sustains the operation of a commercially viable social system.     

青少年文化消费行为对传统家庭的影响——以手机媒体为背景的探讨与研究

从20世纪后半期开始,印刷术时代没落,随之而来的是电视、广播、互联网乃至手机媒体的蒸蒸日上。以手机为代表的新媒体开始以全时、全速、全域、全民的特点充斥在快速成长的青少年生活中,青少年作为主流文化消费者在文化消费中所表现出来追求时尚、注重体验、忽略发展性消费的特点在一定程度上对传统文化家庭的消费决策产生影响。这种影响从根本上不可逆转地改变了传统家庭交流方式,家长被青少年感染,变身孩童化大人,青少年在相对自由的环境中真正得到了身份认同。由此看来,青少年的流行风格与传统家庭文化的碰撞,技术的发展和创新有利于推动传统文化机制的现代化创新。     

经济迷雾中的媒体投放“GPS”

近年，随着国民经济的迅猛发展．中国的消费环境及消费的人群亦发生着剧烈的变化。消费选择不断扩大，生活方式和态度，消费意识和行为亦呈现多样化的趋向。在经济低迷时期．理性节制的态度普遍可见。那么在营销预算收紧时，面对花钱谨慎又谨慎的消费者，企业营销人员怎样才能进行准确有效的媒体投放以提高其ROI？     

走进混媒时代的快消品营销

对于中国这个特殊的市场来说，我们已经可以很明确地感受到：分类营销尽管分类复杂，但对于中国快消品广告主而言。掌握了更多更新的媒体资源，就意味着市场的开拓。