低温碱性蛋白酶芽孢杆菌的筛选鉴定及酶学性质研究

本研究从学院食堂附近的土样、人工湖水样和鲫鱼、鲶鱼的肠道中分离得到6株产蛋白酶菌株。经摇瓶发酵复筛后,选择产酶活高的菌株2、菌株5和菌株6进行形态学观察、生理生化鉴定和部分酶学性质研究。结果表明菌株2可能为短短芽孢杆菌,菌株5可能为苏云金芽孢杆菌,菌株6可能为地衣芽孢杆菌。研究3株菌所产蛋白酶的最适pH,发现2号、5号和6号菌株的最适pH分别为6.0、8.0和10.0。菌株5和菌株6所产蛋白酶在pH为8.0~10.0的缓冲液中处理30min后活性几乎不变,在碱性环境中均具有较好的稳定性。菌株5和菌株6所产蛋白酶的最适反应温度均为30℃,低于30℃处理30min活性几乎不变;50℃处理30min后,菌株5和菌株6的残余酶活分别20%和40%。     

分析产维生素B6乳酸菌的筛选及其鉴定

目的:探讨产维生素B6乳酸菌的筛选及其鉴定情况。方法:选择本地某医院内2~3岁幼儿粪便中分离出的6株乳酸杆菌为研究菌株,分析各菌株所属菌属及其维生素B6产量,并筛选出产维生素B6能力最强的菌株,对其属内种进行鉴定。结果:6株乳酸杆菌,经鉴定均为乳酸菌的乳杆菌属。6株乳酸杆菌均可产生维生素B6,但是产量存在差异,乳酸杆菌N6产量最高,显著高于N1、N2、N3、N4和N5,差异具有统计学意义(P<0.05)。糖发酵试验证实乳酸杆菌N6能发酵葡萄糖(不产气),属同型乳酸发酵类型,同时符合植物乳杆菌鉴定标准。结论:乳酸杆菌具有产生维生素B6的能力,但是产量存在一定差异,而属内种为植物乳杆菌者具有较强的产生维生素B6能力。     

传统酱菜中降解亚硝酸盐枯草芽孢杆菌的筛选和鉴定

酱菜是我国传统风味的调味佳品,但在酱菜的生产过程中易产生大量的亚硝酸盐,因此从酱菜中分离、筛选出能降解亚硝酸盐的高效菌株尤为重要.本实验利用传统分离纯化方法从酱菜中筛选出的能高效降解亚硝酸盐的菌种,并通过现代分子生物学鉴定方法对其进行分析.结果表明,该筛选出的菌株具有一定的降解亚硝酸盐能力,通过分子生物学鉴定为枯草芽孢...     

猪链球菌的实验室分离与鉴定

随着我国国民经济水平的进一步提高,人们对生活的质量以及健康的饮食也越来越关注了.但是近些年来,相关部门在对很多地区的规模猪场进行防疫检查,发现有很多猪身上都带有猪链球菌,如果这些生猪流入市场,被人们所食用的话,那么其后果将会是不堪设想的,下面就对猪链球菌进行分离的细菌进行实验鉴定,以此来保证人们的饮食健康.     

全自动定氮仪-液相色谱测定食品中丙酸

目的：建立操作简单、准确稳定、快速高效测定食品中丙酸的方法。方法：利用全自动定氮仪快速蒸馏的特点,对样品前处理过程中蒸馏试样初始体积、磷酸加入体积、馏出液是否补加磷酸等可能影响测定结果的因素进行逐一验证核实,优化高效液相色谱的样品前处理方法,并用于实际样品的测试。结果：面包和豆腐的方法检出限可以达到0.1 g·kg^-1,当加标浓度为2 g·kg^-1时,面包的加标回收率为76.08%～83.98%,相对极差约为4.9%;豆腐的加标回收率为76.75%～83.24%,相对极差约为4.1%。结论：该方法操作简单快速、结果准确稳定,可以用于面包和豆腐等食品中丙酸的测定。     

宁夏牛奶产业自主创新问题研究

调查了当前宁夏牛奶产业自主创新的现状及存在的技术瓶颈,研究了宁夏牛奶产业自主创新发展战略,提出了解决宁夏牛奶产业发展瓶颈问题的解决方案。     

Attitudes and Preferences of Kosovar Consumer Segments Toward Quality Attributes of Milk and Dairy Products

ABSTRACT

The dairy market is one of the fastest growing agri-food sectors in Kosovo, yet the farm structure is fragmented. The level of productivity is lower compared with European Union levels and shows potential for improvements. The consumption of dairy products is expected to rise due to income growth and segmentation of consumers that would reflect different preferences for low-fat or zero-fat products, products with natural additives, and so on. On the market level, a significant share of the consumed milk and dairy products is imported. This study aims to better understand attitudes and preferences of Kosovar consumer segments toward milk and dairy products. A quantitative survey with 300 consumers was conducted in major Kosovo cities. Applying a reduced version of the Food Related Lifestyle instrument identified three distinct consumer segments: the conservative consumer segment, the socially oriented foodie, and the information-seeking eco-consumer. The latter two segments are especially open to trying new food products and actively search information about food. All three consumer segments show strong consumer patriotism by believing that domestic milk and cheese are safer than imported products and are of higher quality. This indicates the strong market potential for dairy farmers and companies in the Kosovo, which should develop new food products having these segments in mind. The authors explore the strategies that farmers and companies should use to better target these consumer groups and gain greater access to desirable segments.     

Consumer Behavior Toward Goat Milk and Its Processed Products in Malang,Indonesia

The study was carried out in Malang East Java, Indonesia. The purpose was to investigate how consumers behave with regard to fresh goat milk or its processed products and to examine determinants that influence this behavior. One hundred respondents were selected using a convenience sampling method and interviewed using a structured questionnaire. Logistic regression analysis was used to analyze consumer behavior toward fresh goat milk or its processed products. This study could contribute to a better understanding of consumers' demand toward this food. Consumers often purchased fresh goat milk or its processed products whereas only a small number rarely bought this food. Consumers frequently demanded fresh goat milk or its processed products as they had more family members and perceived the importance of the availability, many choices, and the affordable price of this food. On the contrary, consumers with high education and concern about the importance of income, the existence of cow milk and its product, and consumers' perception about fresh goat milk and its processed products as special foods might seldom purchase these foods.     

二噁英的理化性质及在乳及乳制品中污染的研究进展

本文主要分析了二噁英的理化性质,重点介绍了乳及乳制品中二噁英的污染情况,并提出有效措施给予预防和解决,以有效降低二噁英在乳及乳制品中的危害性,确保乳及乳制品行业的健康发展.通过对二噁英的理化性质进行研究,为乳及乳制品的安全生产提供可靠保障,以实现经济与社会效益的最大化.     

液态奶加工中的“过度加热”倾向分析

近年来,我国牛奶加工产业有了迅猛发展,但也存在一些问题,如液态奶加工中的“过度加热”问题。本文通过对过度加热液态奶的判定模式进行探究,分析“过度加热”的危害,总结在液态奶加工中出现“过度加热”的原因,以促进液态奶合理加工,推动我国乳业健康发展。     

大学生奶茶知识、态度和行为(KAP)调查

目的:了解大学生奶茶知识、态度和行为(KAP)现状及存在问题,并探索其影响因素,为有针对性地指导大学生奶茶合理消费提供科学依据.方法:采用调查问卷的方式,调查在校大学生奶茶KAP现况,并对结果进行统计分析.结果:大学生奶茶KAP得分普遍偏低.知识得分中,女大学生高于男大学生、上海地区高于非上海地区大学生、医学类高于非医...     

Consumer Acceptance of Camel Milk in Emerging Economy

Abstract

Camel is considered as an indispensable part of desert eco-framework across the globe. Apart from helping in transport, it is also used for its milk. Camel's milk is used worldwide because of its salty taste. However, it has many beneficial effects such as cure for autism and helps to control diabetes, allergy, and also prevents liver cirrhosis. Objective of this study is to analyze the ways to make people purchase and consume camel milk. In the last few years, awareness about nutritional and medicinal benefits of camel milk has rapidly increased. Consequently, the demand of the product has also increased. Objective of this article is to analyze the consumers’ willingness to buy camel milk before and after knowing its health benefits. It also explores the linkage between lifestyle diseases and consumer’s willingness to buy. We analyzed data in two stages. Initially, we did qualitative analysis with twitter text mining to understand customer’s sentiments about camel milk. Later, primary data was collected through structured questionnaire from a sample of 120 respondents by convenience sampling for quantitative analysis. There is a significant increase in the proportion of people willing to buy camel milk and once they were aware of the benefits, people are willing to buy more after knowing the benefits. People exposed to lifestyle diseases are more willing to buy camel milk. Hence, support of market-based awareness campaigns to stimulate demand for quality camel milk among consumers can be realized through information dissemination and awareness campaign.     

Threshold Cointegration in the Greek Milk Market

This article investigates the nonlinear adjustment between consumer and producer prices in the Greek milk sector using a threshold error correction autoregressive model. The results reject the null hypothesis of linear cointegration in favor of a 2-regime threshold cointegration model. A cointegrating relationship is expected only when equilibrium consumer price is decreased more than 24.12% or the equilibrium relative markup is squeezed more than 62.74%. In this case consumer prices have to increase faster than producer prices to restore the long-run equilibrium between consumer-producer milk prices.     

胶体金免疫层析法检测牛奶中的环丙沙星残留

环丙沙星是恩诺沙星的代谢产物,伴随恩诺沙星在畜牧养殖中广泛应用,从而导致奶牛生产出来的牛奶中可能存在环丙沙星残留.为保障人们的身体健康,监测牛奶环丙沙星残留情况十分必要.本研究试图借助胶体金免疫层析法实现对牛奶环丙沙星残留的检测,结果表明,胶体金免疫层析法可以用来快速检测牛奶中的环丙沙星残留,值得在现场快速筛查中推广使...