The integration of the Internet and the real economy has propelled the formation of a new economy. Internet cultural industries—defined as a collection of industries that engage in creative cultural content, production, circulation, and services based on Internet technology and core digitization—are a critical part of this new economy. This study argues that the development of Internet cultural industries has created a new entrepreneurial ecosystem, which is a dynamic and open ecosystem impacted by numerous factors. Using a structural equation model (SEM) method, we empirically examine the factors impacting the entrepreneurial ecosystem of Internet cultural industries. We find that both external ecosystem factors, including politics, the economy, sociocultural activities, and technological environments, and internal ecosystem factors, including netizens, governments, enterprises, and media and industry associations, are verified to significantly affect the entrepreneurial ecosystem performance of Internet culture industries. Most interestingly, we find that the political environment is the most crucial factor impacting the entrepreneurial ecosystem performance of Internet cultural industries. Thus, Internet cultural industries should prioritize the improvement of good government governance. Overall, our study deepens the research on the Internet entrepreneurial ecosystem by identifying its internal and external determining factors in the context of emerging economies.