| 东北龙胆组织培养繁殖技术研究 |
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| 引用本文： | 李黎. 东北龙胆组织培养繁殖技术研究[J]. 国土与自然资源研究, 2016, 0(4): 88-89. DOI: 10.3969/j.issn.1003-7853.2016.04.028 |
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| 作者姓名： | 李黎 |
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| 作者单位： | 黑龙江省科学院自然与生态研究所湿地与生态保育国家地方联合工程实验室,哈尔滨,150040 |
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| 摘 要： | 以东北龙胆的茎尖、茎段作为外植体,对其组织培养繁殖技术进行研究,结果表明:诱导不定芽分化的最佳培养基是MS+6-BA2.0mg/L+NAA0.1mg/L,分化率可达96.7%,最佳生根培养基为1/2MS+IBA0.3 mg/L,生根率可达97.5%,组培苗最适合的移栽基质为珍珠岩,移栽成活率可达90.0%。
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| 关 键 词： | 东北龙胆 组织培养 激素 培养基 |
The study of propagation techniques by tissue culture of Gentiana manshuica Kitagawa |
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| Abstract: | The study of propagation techniques by tissue culture of stem tips and stem segements of Gentiana manshuica Kitagawa were used as explants. The results showed that the best medium of induction on adventitious bud was MS +6 -BA2.0mg/L +NAA0.1mg/L, and the ratio of differentiation was 96.7%, the best medium of rooting was 1/2MS+ IBA0.3 mg/L, and the ratio of rooting was 97.5%. The best matrix of transplanting of the tissue culture seedling was the perlite, and the ratio of transplanted survival was 90.0%. |
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| Keywords: | Gentiana manshuica Kitagawa Tissue culture Hormone Medium |
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