| 橡胶草叶片愈伤组织诱导研究 |
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| 引用本文： | 陈 菲,曲彦婷,李 黎,等.橡胶草叶片愈伤组织诱导研究[J].国土与自然资源研究,2014(1):93-94. |
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| 作者姓名： | 陈 菲 曲彦婷 李 黎 等 |
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| 作者单位： | [1]黑龙江省科学院自然与生态研究所湿地与生态保育国家地方联合工程实验室,哈尔滨150040 [2]黑龙江省科学院,哈尔滨150040 [3]东北林业大学森林植物生态学教育部重点实验室,哈尔滨150040 |
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| 基金项目： | 黑龙江省科学院预研项目：橡胶草（Tmraxacum kok-saghyz）寒地栽培技术研究 |
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| 摘 要： | 以橡胶草的幼嫩叶片为试验材料,对外植体愈伤组织诱导的优化条件进行了试验研究。试验以MS为基本培养基,以0.1%氯化汞溶液为消毒液,以6-BA和2,4-D为诱导激素,以愈伤组织诱导率和生长状况为筛选指标,不同消毒时间和不同浓度的6-BA和2,4-D对橡胶草愈伤组织诱导的影响进行了试验研究。试验结果表明:6-BA和2,4-D对诱导橡胶草叶片愈伤组织均有影响,最优培养基为MS+6BA 2.0 mg/L+2,4-D 1.0 mg/L,诱导率达83.3%,且愈伤组织生长状况良好。
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| 关 键 词： | 橡胶草 愈伤组织 诱导 |
Study on Leaf Callus Induction of Taraxacum kok-saghyz |
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| Institution: | CHIEN Fei et al (Institute of Natural Resources And Ecology, HAS., National and Provincial Joint Engineering I_aboratory of Wetlands and Ecological Conservation, Harbin 150040, China) |
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| Abstract: | Taking young leaf of Taraxacum kok-saghyz as experimental material, optimum inducing conditions of callus of explant is studied. Taking MS as the basic base, 0.1% content of HgCI solution as disinfectant, 6-BA and 2, 4-D as inducing hormones, inducing rate and growth state as screening indexes, impact of different sterilizing time and different content of 6-BA and 2, 4-D on inducing callus is studied. Results indicate that 6-BA and 2,4-d all have impacts on leaf callus of Taraxacum kok-saghyz, the best culture base is MS ＋ 6BA 2.0 mg/L ＋ 2,4- 1.0 rag/L, with inducing rate of 83.3%, and growth state of callus is well. |
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| Keywords: | Taraxocum kok-saghyz callus induce |
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