| 浅析一株石油降解菌去除石油污染土壤中的油 |
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| 引用本文： | 廖有贵,张小云,欧阳国强,田学达.浅析一株石油降解菌去除石油污染土壤中的油[J].企业技术开发,2007,26(10):3-6. |
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| 作者姓名： | 廖有贵 张小云 欧阳国强 田学达 |
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| 作者单位： | 湘潭大学化工学院，湘潭大学化工学院，湘潭大学化工学院，湘潭大学化工学院 湖南湘潭411105 湖南石油化工职业技术学院，湖南岳阳414012，湖南湘潭411105，湖南湘潭411105，湖南湘潭411105 教授 |
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| 基金项目： | 湘潭大学跨学科交叉研究项目 |
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| 摘 要： | 从某石油厂区附近土样中分离出石油降解菌,经筛选得到13株石油降解较好的真菌。对这13株进行石油降解试验,其中石油降解效果最好的S92对模拟石油降解的降解率为87.0%。以S92菌株为原菌株通过紫外诱变得到一株石油降解能力显著提高的菌株S-3-15。用此菌株对石油污染土样进行石油降解试验,固液质量比为2∶5,接种量为20%,温度为37℃,摇床转速为150r/min,好氧培养了15d,其降解率达到了98.3%。
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| 关 键 词： | 石油 降解 微生物 |
| 文章编号： | 1006-8937（2007）10-0003-04 |
| 修稿时间： | 2007-06-04 |
Screening of epiphyte and its oil degradation ability for petroleum-contaminated soil |
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| LIAO You-gui,ZHANG Xiao-yun,OUYANG Guo-qiang,TIAN Xue-da.Screening of epiphyte and its oil degradation ability for petroleum-contaminated soil[J].Technological Development of Enterprise,2007,26(10):3-6. |
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| Authors: | LIAO You-gui ZHANG Xiao-yun OUYANG Guo-qiang TIAN Xue-da |
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| Institution: | 1. School of Chemical Engineering, Xiangtan University, Xiangtan, Hunan 411105, China;2. Hunan Vocational College of Petrochemical Technology, Yueyang, Hunan 414012, China |
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| Abstract: | Some bacterial strains were separated from the petroleum-contaminated soil taken from an oil fine factory. By a series of screening experiments,13 epiphytes were obtained and used in oil degradation tests. Among them,S92 was the most effective one and got an oil degradation rate of 87.0% on simulation oil-contained soil. S92 was mutagenized to S-3-15 by ultraviolet radiation. The optimum conditions for oil degradation were as follows: ratio of solid to liquid was 2∶5,inoculation capacity was 20%,temperature was 37 ℃,shaking table rotating speed was 150 r/min,and aerobic cultivation for 15 d. Under these conditions,an oil degradation rate of 98.3% was gained by using S-3-15. |
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| Keywords: | petroleum oil degradation microorganism |
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