LPS烟雾干预下的大鼠COPD膈肌细胞凋亡模型研究

使用雄性SPF级Wistar大鼠45只，随机分为A、B、C3组，每组各15只，各组再各分为造模前、造模2周、造模4周3个亚组，每亚组5只．A组正常喂养，B组采用低氧法，C组采用气管注入LPS联合烟雾法造模．采用TUNEL及免疫组织化学法，检测各组膈肌细胞凋亡率及Caspase-3的表达，4周后，经肺HE切片光镜下分析，造模组均符合典型COPD大鼠肺部表现．并计算平均肺泡数（MAN）、肺泡腔与肺总面积比（PAA），A、B、C组膈肌细胞凋亡率及Caspase-3的表达：A组随时间推移，各亚组间无显著差异；B、C组随时间推移，造模2周、4周亚组细胞阳性率显著高于A组，同期亚组（P〈0．01），B、c组内各2周、4周亚组间差异显著（P〈0．05）；B、C造模组间各2周、4周亚组间无统计学差异（P〉0．05）。结果是低氧、烟雾、LPS3种因素都能使大鼠膈肌细胞凋亡，LPS联合烟雾造模组，形成了具有鲜明的COPD大鼠模型特征，更符合COPD大鼠发病机制，为大鼠膈肌细胞凋亡的造模提供了一个选择。

Study of the COPD Model of Diaphragmatic Muscle Cell Apoptosis in Rats by LPS Effect through Inhaling Smoke

Methods: Forty-five male Wistar rats were randomly dived into three equal groups,Control group(A group),Hypoxia(B group),Smoke and LPS group(C group).Then each group was further divided into three subgroups of pre-replication,replication 2 w,4 w subgroups(5 rats each).A group was of normal feeding,B group by inhaling 8% oxygen and C group by inhaling smoke and injecting LPS into tracheas to replicate the models.MAN,PAA was calculated by HE staining clips.Apoptosis and Caspase-3 expression of diaphragmatic m...