| miR-652对非小细胞肺癌H460细胞凋亡和迁移的影响 |
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| 引用本文： | 王建宇,马文俊,王莹. miR-652对非小细胞肺癌H460细胞凋亡和迁移的影响[J]. 中国药物经济学, 2022, 0(1): 44-46,55 |
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| 作者姓名： | 王建宇 马文俊 王莹 |
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| 作者单位： | 牡丹江医学院基础医学院 |
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| 基金项目： | 黑龙江省省属高等学校基本科研业务费科研项目(2017-kyywfmy-0679)。 |
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| 摘 要： | 目的 观察miR-652对非小细胞肺癌H460细胞凋亡和迁移的影响.方法 应用细胞计数实验检测miR-652的浓度对肺癌H460细胞的影响;应用细胞划痕实验检测miR-652对肺癌H460细胞迁移的影响;应用蛋白质免疫印迹技术检测肺癌H460细胞凋亡蛋白表达情况.结果 在H460细胞中,miR-652表达显著减少;转染...
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| 关 键 词： | 非小细胞肺癌 miR-652 H460细胞 Bax 迁移 凋亡 |
Effects of miR-652 on Apoptosis and Migration of Non-small Cell Lung Cancer H460 Cells |
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| WANG Jian-Yu,MA Wen-Jun,WANG Ying. Effects of miR-652 on Apoptosis and Migration of Non-small Cell Lung Cancer H460 Cells[J]. China Journal of Pharmaceutical Economics, 2022, 0(1): 44-46,55 |
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| Authors: | WANG Jian-Yu MA Wen-Jun WANG Ying |
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| Affiliation: | (Basic Medical College,Mudanjiang Medical College of Mudanjiang Medical University,Mudanjiang 157011,China) |
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| Abstract: | Objective To observe the effects of miR-652 on apoptosis and migration of non-small cell lung cancer(NSCLC)H460 cells.Methods The cell counting experiment was used to detect the effect of miR-652 concentration on lung cancer H460 cells;the cell scratch test was used to detect the effect of miR-652 on lung cancer H460 cell migration;the western blot technique was used to detect the expression of apoptotic protein in lung cancer H460 cells.Results In H460 cells,the expression of miR-652 was significantly reduced;after miR-652 was transfected,the cell count of H460 decreased,the cell migration rate decreased,and the expression of Bax and caspase-9 proteins in the cells increased significantly.Conclusion Overexpression of miR-652 inhibits the migration of H460 lung cancer cells and promotes their apoptosis. |
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| Keywords: | Non-small cell lung cancer miR-652 H460 cells Bax Migration Apoptosis |
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