| PMA—PCR技术在植物病原细菌检疫中的应用前景 |
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| 引用本文： | 刘鹏,牛春敬,胡佳续,冯洁,王金成,黄国明. PMA—PCR技术在植物病原细菌检疫中的应用前景[J]. 检验检疫科学, 2014, 0(4): 72-74 |
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| 作者姓名： | 刘鹏 牛春敬 胡佳续 冯洁 王金成 黄国明 |
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| 作者单位： | 天津出入境检验检疫局,天津300461 |
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| 基金项目： | 天津出入境检验检疫局科研课题（TK0030-2013） |
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| 摘 要： | 介绍了PMA-PCR技术检测活菌的方法，列举了该方法在食品、医疗和环境微生物检测领域的应用研究情况，并讨论了PMA．PCR技术在植物病原细菌检疫工作中的应用前景。
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| 关 键 词： | PMA—PCR 病原细菌 检疫 |
The Applications Prospects of PMA-PCR Technology in Plant Pathogenic Bacteria Quarantine |
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| Liu Peng,Niu Chunjing,Hu Jiaxu,Feng Jie,Wang Jincheng,Huang Guoming. The Applications Prospects of PMA-PCR Technology in Plant Pathogenic Bacteria Quarantine[J]. Inspection and Quarantine Science, 2014, 0(4): 72-74 |
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| Authors: | Liu Peng Niu Chunjing Hu Jiaxu Feng Jie Wang Jincheng Huang Guoming |
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| Affiliation: | (Tianjin Entry-Exit Inspection and Quarantine Bureau, Tianj in, 300461) |
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| Abstract: | Propidium monoazide （PMA） is a kind of photosensitive dye which can pass through the damaged cell membrane and has high affinity with DNA molecules. The cross-linking of PMA and DNA can inhibit PCR amplification reactions. The paper describes the introduction of PMA-PCR method which detects viable cells and discusses the application prospects of the method in plant pathogenic bacteria quarantine. |
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| Keywords: | PMA-PCR Pathogenic Bacteria Quarantine |
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