| 黄曲霉毒素前处理方法的研究——免疫亲和柱的重复利用 |
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| 引用本文： | 李佩暖,曹鹏,耿金培,尹大路,阮新.黄曲霉毒素前处理方法的研究——免疫亲和柱的重复利用[J].检验检疫科学,2009,19(5):21-24. |
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| 作者姓名： | 李佩暖 曹鹏 耿金培 尹大路 阮新 |
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| 作者单位： | 烟台出入境检验检疫局,山东烟台,264000 |
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| 基金项目： | 山东出入境检验检疫局科技计划项目 |
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| 摘 要： | 本文对高效液相色谱检测黄曲霉毒素的前处理方法进行优化,建立了免疫亲和柱的回收纯化处理方法。使用过的免疫亲和柱分别用甲醇/水、水冲洗,再用Pbs缓冲溶液进行填充,放入冰箱内于2-8℃保存。对回收柱进行试验,回收率、精密度均达到检测要求,且柱体可以重复使用两次。
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| 关 键 词： | 黄曲霉毒素 免疫亲和柱 回收率 |
Study of the Aflatoxins Sample Preparation-Repeated Usings of Immunoaffinity Columns |
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| Li Peinuan,Cao Peng,Geng Jinpei,Yin Dalu,Ruan Xin.Study of the Aflatoxins Sample Preparation-Repeated Usings of Immunoaffinity Columns[J].Inspection and Quarantine Science,2009,19(5):21-24. |
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| Authors: | Li Peinuan Cao Peng Geng Jinpei Yin Dalu Ruan Xin |
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| Institution: | (Yantai Entry- Exit Inspection and Quarantine Bureau, Yantai, Shandong, 264000) |
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| Abstract: | The sample preparation of aflatoxins by high performance liquid chromatography （HPLC） was improved in this study, and the recovery and purification method for immunoaffinity columns was established. The used immunoaffnity columns were washed by methanol/water, water sequentially, then were filed by Pbs buffer and put into the refrigerator to preserve at 2 - 8℃. After treatments, the immunoaffinity columns can be reused twice with high recovery and good precision. |
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| Keywords: | Aflatoxin Immunoaffinity columns Recoveries |
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