| 姜黄素提取条件的正交设计及硅胶柱色谱纯化的研究 |
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| 引用本文： | 张玉领,陈培,王季茹,陈汉娟,孙长颢.姜黄素提取条件的正交设计及硅胶柱色谱纯化的研究[J].价值工程,2010,29(16):241-242. |
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| 作者姓名： | 张玉领 陈培 王季茹 陈汉娟 孙长颢 |
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| 作者单位： | 1. 淮阴卫生高等职业技术学校,淮安,223300
2. 哈尔滨医科大学,哈尔滨,150000 |
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| 摘 要： | 目的:优化姜黄药材中总姜黄素的提取和纯化最佳工艺条件。方法:采用正交设计优化乙醇回流法提取中药材姜黄中姜黄素的条件,然后以氯仿-甲醇溶液为洗脱液,采用硅胶柱色谱分离法对姜黄素提取液进一步纯化,以提取物得率、总姜黄素含量和纯度为考察指标。采用HPLC法对姜黄素提取液、纯化后的总姜黄素含量、纯度进行检测。结果:通过正交设计发现温度90℃,乙醇浓度75%,料液比10倍,提取时间3h提取率最高;采用硅胶柱色谱分离法对姜黄素提取液进一步纯化后纯度可以达到81.8%。结论:该提取条件下提取工艺合理、科学。
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| 关 键 词： | 姜黄素 乙醇回流法 提取 纯化 HPLC |
Orthogonal Design of Curcumin Extraction Process and Purification of Silica Gel Column Chromatography |
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| Zhang Yuling,Chen Pei,Wang Jiru,Chen Hanjuan,Sun Changhao.Orthogonal Design of Curcumin Extraction Process and Purification of Silica Gel Column Chromatography[J].Value Engineering,2010,29(16):241-242. |
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| Authors: | Zhang Yuling Chen Pei Wang Jiru Chen Hanjuan Sun Changhao |
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| Institution: | ① Zhang Yuling;① Chen Pei;① Wang Jiru;① Chen Hanjuan;② Sun Changhao(①Huaiyin Health Higher Vocational and Technical School,Huaian 223300,China;②Harbin Medical University,Harbin 150000,China) |
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| Abstract: | OBJECTIV：To optimize the extraction and purification process of curcumin.METHODS：The optimum extraction of ethanol refluxing method conditions were investigated by orthogonal design and purification of curcumin solution by silica gel column chromatography method with chloroform-methanol solution,and the content of curcumin was detectd by HPLC.RESULT：The optimum extraction condition obtained was：75% ethanol,temperature of 90℃ with the volume of 10 folds of curcuma longa,refluxing and extracting for 3 hours.After purification by gel column chromatography method,the purity of curcumin powder was 81.8%.CONCLUSION：The optimized process is feasible for the extraction and purification of curcuma longa. |
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| Keywords: | HPLC |
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