| 进出口食品中沙门氏菌PFGE分型及耐药研究 |
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| 引用本文： | 许龙岩,袁慕云,刘静宇,赵贵明,邹志飞,张旺,阳静,焦红.进出口食品中沙门氏菌PFGE分型及耐药研究[J].检验检疫科学,2010,20(2):14-17. |
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| 作者姓名： | 许龙岩 袁慕云 刘静宇 赵贵明 邹志飞 张旺 阳静 焦红 |
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| 作者单位： | 1. 广东出入境检验检疫局,广东广州,510623
2. 中国检验检疫科学研究院 |
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| 基金项目： | 国家质检总局科科技计划项目，广东出入境检验检疫局科技计划项目 |
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| 摘 要： | 目的：研究进出口食品中分离的沙门氏菌（SA）分子型别和耐药情况。方法：用限制性内切酶XbaⅠ酶切SA染色体DNA进行脉冲场凝胶电泳（PFGE）实验,用BioNumerics软件分析菌株之间的相似度。用Vitek 2进行药敏实验,分析SA耐药情况。结果：24株SA分成22个PFGE型。SA2和SA11之间相似度100%,鼠伤寒SA之间的相似度62.1%～90.3%。药敏实验结果显示5株鼠伤寒SA共有4种耐药谱,部分菌株对青霉素类、呋喃类、氨基糖苷类、磺胺类耐药。结论：进出口食品中分离的SA表现出较大的遗传多样性,但部分菌株有相关性。
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| 关 键 词： | 沙门氏菌 脉冲场凝胶电泳 分子分型 食品 耐药 |
Pulsed-field Gel Electrophoresis Typing and Drug Resistance Study of Salmonella |
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| Xu Longyan,Yuan Muyun,Liu Jingyu,Zhao Guiming,Zou Zhifei,Yang Jing,Jiao Hong.Pulsed-field Gel Electrophoresis Typing and Drug Resistance Study of Salmonella[J].Inspection and Quarantine Science,2010,20(2):14-17. |
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| Authors: | Xu Longyan Yuan Muyun Liu Jingyu Zhao Guiming Zou Zhifei Yang Jing Jiao Hong |
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| Institution: | Xu Longyan1,Yuan Muyun1,Liu Jingyu1,Zhao Guiming2,Zou Zhifei1,Yang Jing1,Jiao Hong1 (1.Guangdong Entry-Exit Inspection , Quarantine Bureau,Guangzhou,Guangdong,510623,2.Chinese Academy of Inspection , Quarantine) |
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| Abstract: | The molecular types and drug resistance patterns of Salmonella strains isolated from imported and exported foods were studied.The Salmonella chromosomal DNA was digested by restriction endonuclease XbaI and analyzed by pulsed-field gel electrophoresis(PFGE).The PFGE patterns of Salmonella strains from different areas were compared using BioNumberics software to analyze the similarity between strains.The antibiotic susceptibility test was carried out using VITEK 2 to analyze the drug resistance pattern.The 2... |
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| Keywords: | Salmonella Pulsed-field gel electrophoresis Molecular typing Food Drug resistance |
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