| 高效液相色谱法测定禽蛋中的三聚氰胺 |
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| 引用本文： | 廖峰,赵立军,林顺全,张静.高效液相色谱法测定禽蛋中的三聚氰胺[J].检验检疫科学,2010,20(3):6-8. |
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| 作者姓名： | 廖峰 赵立军 林顺全 张静 |
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| 作者单位： | 农业部畜禽产品质量安全监督检验测试中心,四川成都,610041 |
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| 摘 要： | 本实验建立了禽蛋中三聚氰胺的测定方法。采用高效液相色谱仪,乙腈：（0.01mol/L辛烷磺酸钠和0.01mol/L柠檬酸）=10：90作为流动相,C18色谱柱,检测波长为240nm。结果表明：在0.5-100μg/mL浓度范围,线性关系良好,r=0.9995;变异系数为0.6%;试样测定的相对标准偏差（RSD）〈3%。本法操作简便,测定结果准确。
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| 关 键 词： | 三聚氰胺 禽蛋 高效液相色谱法 |
Determination of Melamine in Eggs by High Performance Liquid Chromatography |
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| Liao Feng,Zhao Lijun,Lin Shunquan,Zhang Jing.Determination of Melamine in Eggs by High Performance Liquid Chromatography[J].Inspection and Quarantine Science,2010,20(3):6-8. |
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| Authors: | Liao Feng Zhao Lijun Lin Shunquan Zhang Jing |
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| Institution: | Liao Feng,Zhao Lijun,Lin Shunquan,Zhang Jing (Test Center for Domestics Animal Quality Supervision and Inspection,Ministry of Agriculture,Chengdu,Sichuan,610041) |
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| Abstract: | A method for determination of melamine in eggs by high performance liquid chromatography(HPLC) was established.The stationary phase was C18 and the mobile phase was acetonitrile:(0.01mol/L citrate and 0.01mol/L sodium octanesulfonate)=90:10(V:V),and the detection wavelength was 240nm.The calibration curve for melamine was linear in the range of 0.5~100ug/mL(r=0.9995),and the relative standard deviation(RSD)<3%.The method is simple,fast and accurate. |
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| Keywords: | Melamine Eggs High Performance Liquid Chromatography(HPLC) |
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