| 菜克多巴胺单克隆抗体的制备及竞争性ELISA检测方法的建立 |
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| 引用本文： | 刘建,梅琳,林祥梅,韩雪清,吴绍强,贾广乐,刘莎,唐慧林.菜克多巴胺单克隆抗体的制备及竞争性ELISA检测方法的建立[J].检验检疫科学,2008,18(5):53-56. |
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| 作者姓名： | 刘建 梅琳 林祥梅 韩雪清 吴绍强 贾广乐 刘莎 唐慧林 |
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| 作者单位： | 中国检验检疫科学研究院,北京,100029 |
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| 基金项目： | 质检总局科研项目:活动物中莱克多巴胺残留快速检测技术研究 |
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| 摘 要： | 本研究利用偶联的莱克多巴胺（Rac）作为免疫原，筛选出能稳定分泌针对莱克多巴胺的单克隆抗体的细胞株D1289，其分泌的单克隆抗体免疫球蛋白亚类为IgCl，单抗腹水的效价不低于1：1×10^7，与Rac的类似物克伦特罗不发生交叉反应。在此基础上，利用获得的单抗研究建立Rac的竞争性酶联免疫吸附检测法（cELISA），结果表明：所建立的间接竞争ELISA方法检测校准曲线的线性范围在0．01ng／mL-10ng／mL（RE=-0．9353）。
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| 关 键 词： | 莱克多巴胺 竞争性ELISA 检测 |
PREPARATION AND c-ELISA OF MONOCLONAL ANTIBODIED AGAINST RACTOPAMINE |
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| LIU Jian,MEI Lin,LIN Xiangmei,HAN Xueqing,WU Shaoqiang,JIA Guangle,LIU Sha,TANG Huilin.PREPARATION AND c-ELISA OF MONOCLONAL ANTIBODIED AGAINST RACTOPAMINE[J].Inspection and Quarantine Science,2008,18(5):53-56. |
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| Authors: | LIU Jian MEI Lin LIN Xiangmei HAN Xueqing WU Shaoqiang JIA Guangle LIU Sha TANG Huilin |
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| Institution: | LIU Jian,MEI Lin,LIN Xiangmei,HAN Xueqing,WU Shaoqiang,JIA Guangle,LIU Sha,TANG Huilin (Chinese Academy of Inspection , Quarantine,Beijing,100029,China) |
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| Abstract: | Our research aimed to find a better means to detect the Ractopamine residue in forage and food. A cell line, D12B9, which secretes monoclonal antibodies against Ractopamine stably, was obtained. The monoclonal antibody characterized by IgG 1 isotype showed no cross-reactivity to Clenbuterol. The ELISA titers of ascites were exceeded 1：1 × 10^7. An indirect competitive ELISA was founded base on the monoclonal antibody. The results ofcELISA showed the linear range of the calibration curves （R2=0.9353） was from 0.01 ng/mL to 10 ng/mL. |
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| Keywords: | Ractopamine Competitive ELISA Detection |
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