| HPLC测定复方菊陈颗粒中绿原酸的含量 |
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| 引用本文： | 黄媚,刘喜华.HPLC测定复方菊陈颗粒中绿原酸的含量[J].中国药物经济学,2012(1):20-24. |
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| 作者姓名： | 黄媚 刘喜华 |
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| 作者单位： | 1. 广西梧州制药集团（股份）有限公司,广西梧州,543002
2. 广西卫生职业技术学院,广西南宁,543002 |
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| 摘 要： | 目的运用HPLC建立复方菊陈颗粒中绿原酸的含量测定方法。方法色谱柱Ultimate XB-C18(4.6×250mm,5μm),乙腈-0.1%磷酸梯度洗脱,流速1ml/min,检测波长328nm,柱温30℃。结果绿原酸在0.066μg～1.652μg范围内呈良好的线性关系(r=0.9998),平均回收率为98.80%,RSD为1.88%(n=6)。结论该方法简便、准确、专属性强,可作为检测复方菊陈颗粒中绿原酸的方法。
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| 关 键 词： | 复方菊陈颗粒 绿原酸 HPLC |
Determination of Chlorogenic acid in Compound Ju Chen Granules by HPLC |
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| HUANG Mei
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LIU Xihua.Determination of Chlorogenic acid in Compound Ju Chen Granules by HPLC[J].China Journal of Pharmaceutical Economics,2012(1):20-24. |
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| Authors: | HUANG Mei LIU Xihua |
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| Institution: | HUANG Mei, LIU Xihua |
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| Abstract: | Objective To establish a method for determination of the content of chlorogenic acid in Compound Ju Chen granules by HPLC.Methods A HPLC method was used with Ultimate XB-C18 column（4.6 mm×250 ram, 5μm ）. The mobile phase was consisted of acetonitrile-0.1% phosphoric acid. The UV detector wavelength was at 328 nm. The flow rate was 1 mL.min-l. The temperature of the column was at 30 ℃ .Results The calibration curve of chlorogenic acid was linear range of 0.066μg-1.652μg （r=0.9998）. The average recovery was 98.80%, RSD=1.88% （n=6）.Conclusion The method is simple, convenient and reliable. It is appropriate for the content determination of the preparation. |
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| Keywords: | Compound Ju Chen Granules Chlorogenic acid HPLC |
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