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艾叶水溶性部位及其分离组分的抑菌活性研究
引用本文:尹美珍,张倩,王静晖,彭玲,喻昕. 艾叶水溶性部位及其分离组分的抑菌活性研究[J]. 黄石理工学院学报, 2011, 27(5): 44-48. DOI: 10.3969/j.issn.1008-8245.2011.05.011
作者姓名:尹美珍  张倩  王静晖  彭玲  喻昕
作者单位:黄石理工学院医学院,湖北黄石,435003
基金项目:黄石市科技局项目,湖北省教育厅重点项目
摘    要:为考察艾叶水溶性部位及其分离组分对金黄色葡萄球菌、大肠杆菌、肺炎克雷伯菌、土生克雷伯菌、铜绿假单胞菌、伤寒杆菌6种供试菌的抑制作用,通过K—B法和比浊法研究总水提物的抑菌活性,采用二分法测定总水提物的最小抑菌浓度、最小杀菌浓度,采用比浊法测定水提物各分离组分的抑菌率。结果为:艾叶总水提物对6种供试菌均有抑制作用,对伤寒杆菌的作用最强;艾叶总水提物分离组分对供试菌有不同程度的抑制作用,其中硅胶柱(无水乙醇:三乙胺=1000:1)洗脱物对供试菌的抑菌活性最强。艾叶水提物及其分离组分均具有较强的抑菌活性。

关 键 词:艾叶  萃取  抑菌活性  抑菌率

Study on Antimicrobial Activities of Aqueous Extract from Balanophora Japonica
YIN Meizhen,ZHANG Qian,WANG Jinghui,PENG Ling,YU Xin. Study on Antimicrobial Activities of Aqueous Extract from Balanophora Japonica[J]. Journal of Huangshi Institute of Technology, 2011, 27(5): 44-48. DOI: 10.3969/j.issn.1008-8245.2011.05.011
Authors:YIN Meizhen  ZHANG Qian  WANG Jinghui  PENG Ling  YU Xin
Affiliation:* (School of Medicine,Huangshi Institute of Technology,Huangshi Hubei 435003)
Abstract:Objectives:To investigate the inhibition role of total aqueous extract(TAC) and its separating components(SCs) from Artemisia argyi on staphylococcus aureus, escherichia coli, klebsiella pneumonia, hebsiella terrigena, pseudomonas aeruginosa and sahnonella typhi. Methods:The antimicrobial activity of TAC from Artemisia argyi was investigated with K - B method and turbidimetric method. The minimum inhibitory and minimum bactericidal concentrations (MIC and MBC) were studied by bisection method. The bacterio- stasis rate of SCs was studied with turbidimetric method. Results: TAC showed strong and wide antibacterial activity. Different SCs showed different bacteriostasis, in which elute from silica gel( eluted with ethanol :tri- ethylamine =1 000 : 1 ) had the strongest antibacterial activity. Conclusion : Both TAC and SCs have antibacterial activity, and further antimicrobial screening and structure identification are under the way.
Keywords:artemisia argyi  extraction  antimicrobial activity  bacteriostasis rate
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