艾叶水溶性部位及其分离组分的抑菌活性研究

为考察艾叶水溶性部位及其分离组分对金黄色葡萄球菌、大肠杆菌、肺炎克雷伯菌、土生克雷伯菌、铜绿假单胞菌、伤寒杆菌6种供试菌的抑制作用，通过K—B法和比浊法研究总水提物的抑菌活性，采用二分法测定总水提物的最小抑菌浓度、最小杀菌浓度，采用比浊法测定水提物各分离组分的抑菌率。结果为：艾叶总水提物对6种供试菌均有抑制作用，对伤寒杆菌的作用最强；艾叶总水提物分离组分对供试菌有不同程度的抑制作用，其中硅胶柱（无水乙醇：三乙胺=1000：1）洗脱物对供试菌的抑菌活性最强。艾叶水提物及其分离组分均具有较强的抑菌活性。

Study on Antimicrobial Activities of Aqueous Extract from Balanophora Japonica

Objectives：To investigate the inhibition role of total aqueous extract（TAC） and its separating components（SCs） from Artemisia argyi on staphylococcus aureus, escherichia coli, klebsiella pneumonia, hebsiella terrigena, pseudomonas aeruginosa and sahnonella typhi. Methods：The antimicrobial activity of TAC from Artemisia argyi was investigated with K - B method and turbidimetric method. The minimum inhibitory and minimum bactericidal concentrations （MIC and MBC） were studied by bisection method. The bacterio- stasis rate of SCs was studied with turbidimetric method. Results： TAC showed strong and wide antibacterial activity. Different SCs showed different bacteriostasis, in which elute from silica gel（ eluted with ethanol ：tri- ethylamine =1 000 ： 1 ） had the strongest antibacterial activity. Conclusion ： Both TAC and SCs have antibacterial activity, and further antimicrobial screening and structure identification are under the way.