| UPLC／MS／MS测定动物组织中4种氟喹诺酮类药物残留量 |
| |
| 引用本文： | 曹鹏,田德金,李佩暖,娄喜山,郭海霞.UPLC／MS／MS测定动物组织中4种氟喹诺酮类药物残留量[J].检验检疫科学,2008,18(4):48-50. |
| |
| 作者姓名： | 曹鹏 田德金 李佩暖 娄喜山 郭海霞 |
| |
| 作者单位： | 1. 烟台出入境检验检疫局,山东,烟台,264000
2. 龙大食品集团检测中心,山东,莱阳,265231 |
| |
| 摘 要： | 本文建立了动物组织中4种氟喹诺酮类药物（恩诺沙星、环丙沙星、氧氟沙星和诺氟沙星）同时检测的超高效液相色谱串联质谱法。采用乙腈提取样品中的药物，然后加入乙腈饱和正己烷脱脂、除杂，提取液浓缩、定容后进超高效液质联用仪（UPLC／MS／MS）检测，外标法定量。方法的回收率为75％-100％，相对标准偏差〈10％．定量限为1μg／kg。
|
| 关 键 词： | 氟喹诺酮类药物 超高效液相色谱 串联质谱 |
DETERMINATION OF FLUOROQUINOLONES RESIDUES IN ANIMAL TISSUES BY UPLC-MS/MS |
| |
| CAO Peng,TIAN Dejin,LI Peinuan,LOU Xishan,GUO Haixia.DETERMINATION OF FLUOROQUINOLONES RESIDUES IN ANIMAL TISSUES BY UPLC-MS/MS[J].Inspection and Quarantine Science,2008,18(4):48-50. |
| |
| Authors: | CAO Peng TIAN Dejin LI Peinuan LOU Xishan GUO Haixia |
| |
| Institution: | CAO Peng1,TIAN Dejin2,LI Peinuan1,LOU Xishan2,GUO Haixia2(1.Yantai Entry-Exit Inspection , Quarantine Bureau,Yantai,Sh,ong,264000,China,2.Test & Measurement Center,Longda Foodstuffs Group Co.,Ltd,265231,China) |
| |
| Abstract: | A UPLC/MS/MS method was developed for the determination of four fluoroquinolones （enrofloxacin, ciproftoxacin, ofloxacin and norfloxacin） in animal tissues. In this method, residues were extracted from samples by acetonitrile, degreased with n-hexane saturated with acetonitrile and purified. After the extracts were concentrated and the volumes were determined, the extracts were injected into the UPLC/MS/MS to detect and quantified by external standard method. The average recovery was 75%-100%, the relative standard deviation was less than 10% and the lower limit for detection was 1μg/kg. |
| |
| Keywords: | Fluoroquinolones UPLC LC/MS/MS |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
